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<p>Composting of biodegradable waste like leaf litter and kitchen waste is an eco-friendly and cost-effective method of management of such waste and producing good-quality compost. For the present study, three types of compost were prepared using different combinations of leaf litter (LL), kitchen waste (KW) and cattle dung (CD) to explore the potential of their aqueous extracts (2.5 to 25.0%) on seed germination and micropropagation in <i>Withania coagulans</i>. Compositions of feed mixtures for three types of compost are as follows: CT1 (Compost type-1): LL (25.0%), KW (50.0%) and CD (25.0%); CT2 (Compost type-2): LL (35.0%), KW (50.0%) and CD (15.0%); and CT3 (Compost type-3): LL (25.0%) and KW (75.0%). Analysis of their physico-chemical parameters revealed that electrical conductivity, total Kjeldahl nitrogen, total available phosphorus, total sodium and total potassium content increased in all types of composts when compared to their feed mixtures while pH, total organic carbon and C/N ratio decreased at the end of the composting period (after 120&#xa0;days). Seed germination study revealed that among all compost treatments, CT1 induced maximum seed germination (84.44%) at 5.0% concentration. Control group induced 31.11% seed germination. During micropropagation studies, aqueous extracts of compost showed maximum shoot regeneration (91.66%) with CT1-15.0%, followed by 84.72% with CT1-5.0%. After 4 wk, regenerated shoots were transferred to fresh medium of same composition for shoot elongation and rooting. Maximum root regeneration percentage (76.38%) was observed with CT1-15.0%, followed by 73.6% with CT2 -5.0%. Plantlets were acclimatized and showed 100% survival rate in outdoor conditions&#xa0;at Guru Nanak Dev University, Amritsar, India.</p>

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Biotransformation of organic waste into compost: Implications for in vitro conservation of Withania coagulans (Stocks) Dunal

  • 期刊论文

  • 发表日期：

    2025年1月13日

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  • Sonali,
  • Anamika Sharma,
  • Jatinder Kaur Katnoria,
  • Avinash Kaur Nagpal

摘要

Composting of biodegradable waste like leaf litter and kitchen waste is an eco-friendly and cost-effective method of management of such waste and producing good-quality compost. For the present study, three types of compost were prepared using different combinations of leaf litter (LL), kitchen waste (KW) and cattle dung (CD) to explore the potential of their aqueous extracts (2.5 to 25.0%) on seed germination and micropropagation in Withania coagulans. Compositions of feed mixtures for three types of compost are as follows: CT1 (Compost type-1): LL (25.0%), KW (50.0%) and CD (25.0%); CT2 (Compost type-2): LL (35.0%), KW (50.0%) and CD (15.0%); and CT3 (Compost type-3): LL (25.0%) and KW (75.0%). Analysis of their physico-chemical parameters revealed that electrical conductivity, total Kjeldahl nitrogen, total available phosphorus, total sodium and total potassium content increased in all types of composts when compared to their feed mixtures while pH, total organic carbon and C/N ratio decreased at the end of the composting period (after 120 days). Seed germination study revealed that among all compost treatments, CT1 induced maximum seed germination (84.44%) at 5.0% concentration. Control group induced 31.11% seed germination. During micropropagation studies, aqueous extracts of compost showed maximum shoot regeneration (91.66%) with CT1-15.0%, followed by 84.72% with CT1-5.0%. After 4 wk, regenerated shoots were transferred to fresh medium of same composition for shoot elongation and rooting. Maximum root regeneration percentage (76.38%) was observed with CT1-15.0%, followed by 73.6% with CT2 -5.0%. Plantlets were acclimatized and showed 100% survival rate in outdoor conditions at Guru Nanak Dev University, Amritsar, India.