Podophyllotoxin bioaccumulation with biotic and abiotic elicitors on root culture of Hyptis suaveolens (L.) POIT
摘要
Hyptis suaveolens is a medicinal and aromatic species that exhibits insecticidal, fungicidal, bactericidal, and nematicidal activity. Its roots contain podophyllotoxin (PTOX), a lignan used by the pharmaceutical industry. Elicitors can stimulate the in vitro production of PTOX. Thus, the aim of this study was to evaluate the use of biotic and abiotic elicitors in the production of PTOX in root culture. Roots of plantlets grown in vitro were excised, weighed, and inserted into Erlenmeyer flasks containing 50.0 mL of Murashige and Skoog (MS) medium and cultivated with different biotic elicitors: melatonin, serotonin, methyl jasmonate, salicylic acid, and chitosan, and abiotic elicitor: light quality. After 42 d, the fresh and dry weight of the roots and the production of PTOX in the roots and medium were assessed. Cultivation under red LED light increased the accumulation of PTOX in the medium and the dry weight of the roots. However, the concentrations of 50.0 and 100.0 mg L−1 of chitosan provided the best results in terms of total PTOX production. The addition of 30.0 µM melatonin was responsible for the highest PTOX production. Elicitation with 10.0 µM of serotonin promoted a higher production of fresh and dry root biomass and better growth indices. Supplementing MS culture medium with 5.0 and 10.0 mM of methyl jasmonate favored root growth while the use of 5.0 mM benefited PTOX production. The addition of 50.0 mM of salicylic acid provided a higher production of PTOX in the roots. The use of biotic and abiotic elicitors influenced the increase in root biomass and PTOX production through root culture.