Scholarlink

<p>Heat stress limits wheat productivity, causing huge yield loss worldwide, especially during the post-anthesis stage. The gene <i>TaTT1</i> is highly upregulated in wheat at the seedling and anthesis stages in the heat stress-tolerant (HD3086) wheat genotype as compared to the heat-sensitive genotype (HD2894). The gene <i>TaTT1</i> was successfully transformed and overexpressed in prokaryotic systems and transgenic <i>Nicotiana tabacum</i> L. (tobacco) plants. Furthermore, in transgenic tobacco lines, the transgene <i>TaTT1</i> was confirmed by performing PCR, dot blot, and Southern hybridization blot analysis. Out of 8 Southern positive lines, only 5 showed single copy insertion of <i>TaTT1</i> transgene. The transgenic lines having a single copy of gene insertion were further studied at the T<sub>1</sub> stage. Different biochemical assays (MDA, proline, chlorophyll content, RWC) and real-time PCR were performed after imposing heat stress on T<sub>1</sub> transgenic tobacco lines. Transgenic tobacco lines could tolerate imposed heat stress more efficiently compared to control (untransformed) tobacco plants. Moreover, the recombinant bacterial cells carrying gene <i>TaTT1</i> performed better under heat stress exposure of 45°C and also revealed through their induction in protein blot. The gene <i>TaTT1</i> could successfully provide heat stress tolerance in plant and prokaryotic model systems. This would assure breeders and researchers to use the gene for wheat crop development, keeping in mind the prevailing and predicted future environmental conditions.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Stress-associated gene TaTT1 from wheat confers heat stress tolerance in the prokaryotic system and transgenic tobacco (Nicotiana tabacum L.)

  • 期刊论文

  • 发表日期：

    2025年1月14日

查看全文
  • Satish Kumar,
  • Harinder Vishwakarma,
  • Kishor Prabhakar Panzade,
  • Nisha Jaiswal,
  • Diwakar Aggarwal

摘要

Heat stress limits wheat productivity, causing huge yield loss worldwide, especially during the post-anthesis stage. The gene TaTT1 is highly upregulated in wheat at the seedling and anthesis stages in the heat stress-tolerant (HD3086) wheat genotype as compared to the heat-sensitive genotype (HD2894). The gene TaTT1 was successfully transformed and overexpressed in prokaryotic systems and transgenic Nicotiana tabacum L. (tobacco) plants. Furthermore, in transgenic tobacco lines, the transgene TaTT1 was confirmed by performing PCR, dot blot, and Southern hybridization blot analysis. Out of 8 Southern positive lines, only 5 showed single copy insertion of TaTT1 transgene. The transgenic lines having a single copy of gene insertion were further studied at the T1 stage. Different biochemical assays (MDA, proline, chlorophyll content, RWC) and real-time PCR were performed after imposing heat stress on T1 transgenic tobacco lines. Transgenic tobacco lines could tolerate imposed heat stress more efficiently compared to control (untransformed) tobacco plants. Moreover, the recombinant bacterial cells carrying gene TaTT1 performed better under heat stress exposure of 45°C and also revealed through their induction in protein blot. The gene TaTT1 could successfully provide heat stress tolerance in plant and prokaryotic model systems. This would assure breeders and researchers to use the gene for wheat crop development, keeping in mind the prevailing and predicted future environmental conditions.