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<p>Because of its unique flower shape, brilliant color, long flowering period, and high commercial value, <i>Anthurium andraeanum</i> Linden is one of the leading potted flowers<i>.</i> Polyploid germplasm resources have important utilization value in the study of genetic evolution of species and the breeding of new varieties. The <i>A. andraeanum</i> industry also needs newer polyploid varieties, which requires more polyploid parental materials. This study was envisaged to obtain high-quality new tetraploid germplasm of <i>A. andraeanum</i> also known as ‘Pink Champion.’ Specifically, the callus of <i>A. andraeanum</i> was treated with 0.5, 1.0, 2.0, and 4.0 g L<sup>−1</sup> of colchicine for 2, 4, 6, and 8 h, respectively, and was then transferred to the culture medium of MS medium + 6-benzylaminopurine 2.0 mg L<sup>−1</sup> + naphthylacetic acid 0.5 mg L<sup>−1</sup>. After 75 d being kept in the same culture vessel, the survival rate of callus and the number of regenerated plants were counted, and the regenerated plants were identified as tetraploid using morphological detection, microscopy, chromosome counting, and flow cytometry. The results showed that the concentration of colchicine was inversely proportional to the survival rate of callus and the number of induced regenerated plants. The highest tetraploid induction rate of 9.34% was achieved when 2.0 g L<sup>−1</sup> colchicine was applied for 6 h. The genomes of <i>A. andraeanum</i> diploid and tetraploid plants were 3.71 and 7.39 Gb, respectively; the karyotypic formula of <i>A. andraeanum</i> diploid plants was 2n = 2x = 30 = 2st + 18sm + 10m, and the karyotype formula for <i>A. andraeanum</i> tetraploid plants was 2n = 4x = 60 = 12st + 40sm + 8m. The tetraploid plants exhibited thicker leaves, larger stomatal apparatus, and smaller guard cell density than the diploids. Furthermore, the flower diameters and flowering period of tetraploid plants were significantly larger than those of diploids. In conclusion, the use of 2.0 g L<sup>−1</sup> colchicine treatment for 6 h can cultivate <i>A. andraeanum</i> tetraploid plants, and the tetraploid plants exhibit larger flowers and longer flowering periods than diploids. The tetraploid plants may have potential market prospects or may be used as promising material for breeding new <i>A. andraeanum</i> germplasm resources such as triploids.</p>

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In vitro induction and characterization of Anthurium andraeanum ‘Pink Champion’ tetraploids

  • 期刊论文

  • 发表日期：

    2025年1月6日

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  • Yongping Zhang,
  • Yongxu Qiao,
  • Weihua Zhang,
  • Xingyi Liu,
  • Rongya Gong,
  • Ziying Wang,
  • Yun Zhang

摘要

Because of its unique flower shape, brilliant color, long flowering period, and high commercial value, Anthurium andraeanum Linden is one of the leading potted flowers. Polyploid germplasm resources have important utilization value in the study of genetic evolution of species and the breeding of new varieties. The A. andraeanum industry also needs newer polyploid varieties, which requires more polyploid parental materials. This study was envisaged to obtain high-quality new tetraploid germplasm of A. andraeanum also known as ‘Pink Champion.’ Specifically, the callus of A. andraeanum was treated with 0.5, 1.0, 2.0, and 4.0 g L−1 of colchicine for 2, 4, 6, and 8 h, respectively, and was then transferred to the culture medium of MS medium + 6-benzylaminopurine 2.0 mg L−1 + naphthylacetic acid 0.5 mg L−1. After 75 d being kept in the same culture vessel, the survival rate of callus and the number of regenerated plants were counted, and the regenerated plants were identified as tetraploid using morphological detection, microscopy, chromosome counting, and flow cytometry. The results showed that the concentration of colchicine was inversely proportional to the survival rate of callus and the number of induced regenerated plants. The highest tetraploid induction rate of 9.34% was achieved when 2.0 g L−1 colchicine was applied for 6 h. The genomes of A. andraeanum diploid and tetraploid plants were 3.71 and 7.39 Gb, respectively; the karyotypic formula of A. andraeanum diploid plants was 2n = 2x = 30 = 2st + 18sm + 10m, and the karyotype formula for A. andraeanum tetraploid plants was 2n = 4x = 60 = 12st + 40sm + 8m. The tetraploid plants exhibited thicker leaves, larger stomatal apparatus, and smaller guard cell density than the diploids. Furthermore, the flower diameters and flowering period of tetraploid plants were significantly larger than those of diploids. In conclusion, the use of 2.0 g L−1 colchicine treatment for 6 h can cultivate A. andraeanum tetraploid plants, and the tetraploid plants exhibit larger flowers and longer flowering periods than diploids. The tetraploid plants may have potential market prospects or may be used as promising material for breeding new A. andraeanum germplasm resources such as triploids.