Scholarlink

<p>To develop a biological pacemaker by differentiating rabbit BMSCs into vascular endothelial cells (VECs) and pacemaker-like cells, and constructing a 3D vascularized sinoatrial node (SAN) in vitro. BMSCs were isolated via whole bone marrow adherence. Sinoatrial node cells were purified using differential adhesion with 5-BrdU treatment, and their lysate was prepared by freeze–thaw cycling. BMSCs were induced into VECs (CD31+/CD34+) using endothelial medium and into pacemaker-like cells (HCN2+/cTnT+) using SAN lysate. These cells were co-cultured on Matrigel at 1:1, 1:2, and 2:1 ratios to form 3D vascularized constructs. Cell distribution was analyzed via frozen sectioning and H&amp;E staining. BMSCs successfully differentiated into VECs and pacemaker-like cells, confirmed by marker expression. The 1:1 co-culture ratio optimally promoted uniform cell distribution, network formation, and angiogenesis in the Matrigel scaffold. This study demonstrates that BMSCs can be differentiated into functional pacemaker-like cells and VECs, enabling the in vitro construction of a vascularized tissue-engineered SAN—a promising step toward biological pacemaker development.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Construction of tissue-engineered vascular sinoatrial node in vitro

  • 期刊论文

  • 发表日期：

    2026年3月23日

查看全文
  • Xiaolong Yu,
  • Qing Chang,
  • Lina Wang

摘要

To develop a biological pacemaker by differentiating rabbit BMSCs into vascular endothelial cells (VECs) and pacemaker-like cells, and constructing a 3D vascularized sinoatrial node (SAN) in vitro. BMSCs were isolated via whole bone marrow adherence. Sinoatrial node cells were purified using differential adhesion with 5-BrdU treatment, and their lysate was prepared by freeze–thaw cycling. BMSCs were induced into VECs (CD31+/CD34+) using endothelial medium and into pacemaker-like cells (HCN2+/cTnT+) using SAN lysate. These cells were co-cultured on Matrigel at 1:1, 1:2, and 2:1 ratios to form 3D vascularized constructs. Cell distribution was analyzed via frozen sectioning and H&E staining. BMSCs successfully differentiated into VECs and pacemaker-like cells, confirmed by marker expression. The 1:1 co-culture ratio optimally promoted uniform cell distribution, network formation, and angiogenesis in the Matrigel scaffold. This study demonstrates that BMSCs can be differentiated into functional pacemaker-like cells and VECs, enabling the in vitro construction of a vascularized tissue-engineered SAN—a promising step toward biological pacemaker development.