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<p>Insect cell lines are valuable for basic and applied biological research. In this study, we established 27 cell lines from various insect species, including Hemiptera: <i>Nilaparvata lugens</i>, Coleoptera: <i>Sitophilus oryzae</i>, Hymenoptera: <i>Allantus luctifer</i> and <i>Trichogramma</i> ssp., Diptera: <i>Culicoides oxystoma</i>, Lepidoptera: <i>Spodoptera litura</i>, <i>Mythimna separata</i><i>, </i><i>Bombyx mori</i>, <i>Agrius convolvuli</i>, <i>Plodia interpunctella</i>, and <i>Cryptophlebia horii</i>. This is the first report of cell lines derived from <i>A. luctifer</i>, <i>C. oxystoma</i>, <i>A. convolvuli</i>, and <i>C. horii</i>. Additionally, cell lines from <i>S. litura</i> and <i>M. separata</i> were established from different tissues including the hemocytes, fat bodies, embryos, and Malpighian tubules. Eighteen cell lines were successfully adapted to commercial culture media, with the population doubling time ranging from 1 to 8 d. The identities of the cell lines were confirmed using DNA barcoding. These established cell lines could be valuable for various research applications.</p>

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Establishment of 27 cell lines derived from various insects

  • 期刊论文

  • 发表日期：

    2025年4月8日

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  • Kazuyo Watanabe,
  • Shigeo Imanishi,
  • Takumi Kayukawa,
  • Ken Tateishi

摘要

Insect cell lines are valuable for basic and applied biological research. In this study, we established 27 cell lines from various insect species, including Hemiptera: Nilaparvata lugens, Coleoptera: Sitophilus oryzae, Hymenoptera: Allantus luctifer and Trichogramma ssp., Diptera: Culicoides oxystoma, Lepidoptera: Spodoptera litura, Mythimna separata, Bombyx mori, Agrius convolvuli, Plodia interpunctella, and Cryptophlebia horii. This is the first report of cell lines derived from A. luctifer, C. oxystoma, A. convolvuli, and C. horii. Additionally, cell lines from S. litura and M. separata were established from different tissues including the hemocytes, fat bodies, embryos, and Malpighian tubules. Eighteen cell lines were successfully adapted to commercial culture media, with the population doubling time ranging from 1 to 8 d. The identities of the cell lines were confirmed using DNA barcoding. These established cell lines could be valuable for various research applications.