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<p>The myoglobin (Mb) biofunctionalized chitosan (Chit) with carboxylic acid functionalized multi-walled carbon nanotubes (FMWCNT) modified polypyrrole (PPy) electrodeposited screen printed carbon electrode (SPCE) showed an excellent biocatalytic activity towards the one-electron redox reaction of nitrite and the reduction of H<sub>2</sub>O<sub>2</sub>. The direct electron transfer of the Mb immobilized into the FMWCNT was greatly facilitated. Each and every step of surface morphological changes as studied by scanning electron microscope (SEM), while electrochemical changes were monitored <i>via</i> cyclic voltammetry (CV). The voltammetric response of the fabricated biosensor platform varied linearly with varying NO<sub>2</sub><sup>–</sup> concentration of 10 to 800 µM by a detection limit of 1 µM. Further, the electrochemical response of the constructed biosensor changed linearly with the H<sub>2</sub>O<sub>2</sub> in the range of 1 to 600 µM, with a detection limit of 0.5 µM. Moreover, a low-cost electrochemical virtual cyclic voltammetric analyzer was developed based on a home-made potentiostat and graphical user-interface software LabVIEW 10.0. The performance of the developed virtual analyzer has been further evaluated by applying it for the measurement of NO<sub>2</sub><sup>–</sup> and H<sub>2</sub>O<sub>2</sub> in L-NAME-treated embryonic rat heart-derived H9c2 cardiomyoblasts, and the results are validated with a commercial electrochemical analyzer.</p>

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Electrochemical biosensor based on myoglobin immobilized chitosan/FMWCNT matrix for simultaneous detection of nitrite and hydrogen peroxide

  • 期刊论文

  • 发表日期：

    2026年7月21日

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  • Paulraj Santharaman,
  • Chandran Karunakaran,
  • Raju Venkatesan,
  • Thesingu Rajan Arun,
  • Athithan Maheshwaran,
  • Thangavel Rajesh Kumar,
  • Chitrarasu Manikandan,
  • Subramani Dorothy

摘要

The myoglobin (Mb) biofunctionalized chitosan (Chit) with carboxylic acid functionalized multi-walled carbon nanotubes (FMWCNT) modified polypyrrole (PPy) electrodeposited screen printed carbon electrode (SPCE) showed an excellent biocatalytic activity towards the one-electron redox reaction of nitrite and the reduction of H2O2. The direct electron transfer of the Mb immobilized into the FMWCNT was greatly facilitated. Each and every step of surface morphological changes as studied by scanning electron microscope (SEM), while electrochemical changes were monitored via cyclic voltammetry (CV). The voltammetric response of the fabricated biosensor platform varied linearly with varying NO2– concentration of 10 to 800 µM by a detection limit of 1 µM. Further, the electrochemical response of the constructed biosensor changed linearly with the H2O2 in the range of 1 to 600 µM, with a detection limit of 0.5 µM. Moreover, a low-cost electrochemical virtual cyclic voltammetric analyzer was developed based on a home-made potentiostat and graphical user-interface software LabVIEW 10.0. The performance of the developed virtual analyzer has been further evaluated by applying it for the measurement of NO2– and H2O2 in L-NAME-treated embryonic rat heart-derived H9c2 cardiomyoblasts, and the results are validated with a commercial electrochemical analyzer.