<p>In the current study, a novel indium oxide nanoparticle (In₂O₃ NPs) is synthesised via the combustion method for effective rutin detection through the voltammetric technique. The phase structure of In₂O₃ NPs is evaluated using X-ray diffraction (XRD), morphological analysis through Field emission scanning electron microscopy (FESEM), and the composition of elements by Energy dispersive X-ray (EDX), and the presence of different modes of vibrational by Raman spectroscopy. The electrode is fabricated using In₂O₃ NPs with carbon powder composite and further modified with the polymerisation of arginine to form (AMICCPE) arginine-modified indium oxide composite carbon paste electrode. This fabricated electrode is utilised to analyse using electrochemical impedance spectroscopy (EIS), linear sweep voltammetry (LSV), cyclic voltammetry (CV), and differential pulse voltammetry (DPV) techniques. The fabricated electrode surface enables the detection of RT at 5.8 pH via diffusion-controlled redox behaviour, with an average detection limit (LOD) of 0.039 µM, good selectivity, and sensitive simultaneous analysis and real samples, yielding recovery rates. The fabricated electrode exhibits excellent sensitivity, selectivity, stability, high reproducibility, and repeatability. The aim is to analyse rutin content in food and pharmaceutical samples as an excellent electroactive material for the electrochemical analysis of rutin (RT).</p>