Scholarlink

<p>Hepatitis B is a viral infection (HBV) that affects millions of people worldwide and causes HBV-related liver diseases, leading to both chronic and acute complications. Patients affected by HBV need a regular monitoring of disease progression and viral activity for a treatment and to reduce the risk. In this research, a highly sensitive HBsAg (hepatitis B surface antigen) aptasensor was developed on an interdigitated microelectrode (IDME). Amine-modified gold nanoparticles (Au NPs) were used as the linker to attach a capture probe of aptamer on IDME. To improve the aptamer immobilization, COOH-ended aptamer was premixed with glutaraldehyde and attached on IDME through the amine-Au NPs. This method of aptamer immobilization increased the number of aptamers on the electrode and detected HBsAg as low as 0.1&#xa0;ng/mL on a linear regression coefficient curve within the range of 0.1 to 2&#xa0;ng/mL. Furthermore, HBsAg-spiked serum increased the response of current without interference of proteins in the serum, indicating a specific detection of HBsAg. Control proteins HBeAg, HBeAb, HBsAb, HbcAb, albumin, and complementary aptamer did not change the current responses, confirming the specific detection of HBsAg by its aptamer. This HBsAg biosensor quantifies lower levels of HBsAg and helps to screen hepatitis B infection.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Diagnosis of hepatitis B virus by an aptasensor developed on a microelectrode

  • 期刊论文

  • 发表日期：

    2025年3月14日

查看全文
  • Jintang Yin,
  • Yuefang Pan,
  • Subash C. B. Gopinath

摘要

Hepatitis B is a viral infection (HBV) that affects millions of people worldwide and causes HBV-related liver diseases, leading to both chronic and acute complications. Patients affected by HBV need a regular monitoring of disease progression and viral activity for a treatment and to reduce the risk. In this research, a highly sensitive HBsAg (hepatitis B surface antigen) aptasensor was developed on an interdigitated microelectrode (IDME). Amine-modified gold nanoparticles (Au NPs) were used as the linker to attach a capture probe of aptamer on IDME. To improve the aptamer immobilization, COOH-ended aptamer was premixed with glutaraldehyde and attached on IDME through the amine-Au NPs. This method of aptamer immobilization increased the number of aptamers on the electrode and detected HBsAg as low as 0.1 ng/mL on a linear regression coefficient curve within the range of 0.1 to 2 ng/mL. Furthermore, HBsAg-spiked serum increased the response of current without interference of proteins in the serum, indicating a specific detection of HBsAg. Control proteins HBeAg, HBeAb, HBsAb, HbcAb, albumin, and complementary aptamer did not change the current responses, confirming the specific detection of HBsAg by its aptamer. This HBsAg biosensor quantifies lower levels of HBsAg and helps to screen hepatitis B infection.