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<p>In this study, we employ integrative taxonomy and multigene phylogenetic analysis to identify and describe three novel species of the genus <i>Clitopilus</i> from southern Punjab, Pakistan. Detailed morphological examinations were conducted alongside advanced phylogenomic techniques, incorporating sequences from multiple genetic loci, including internal transcribed spacer (ITS), large ribosomal subunit (28S), and second largest of RNA polymerase-II (<i>RPB2</i>) regions. Our comprehensive morphological and phylogenetic approach allowed us to delineate distinct morphological traits and unique genetic profiles of the newly identified taxa viz<i>. C. albominimus, C. inops,</i> and <i>C. pirosylvanus.</i> The multigene phylogenetic analysis using maximum likelihood and Bayesian inference methods showed that the three new species form distinct branches, separate from any previously reported <i>Clitopilus</i> species, and placed them within the <i>Clitopilus</i> sect. <i>Scyphoides.</i> This research underscores the importance of integrative methods combining classical taxonomy with genomic tools to elucidate fungal biodiversity. Our findings not only expand the known diversity of <i>Clitopilus</i> in Pakistan but also provide valuable insights into the evolutionary relationships within the genus, paving the way for future studies on their ecological roles and conservation needs.</p>

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Integrative taxonomy and multigene phylogeny uncover three novel taxa of the genus Clitopilus from southern Punjab, Pakistan

  • 期刊论文

  • 发表日期：

    2025年4月11日

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  • Muhammad Asif,
  • Malka Saba,
  • Wajahat Akram,
  • Fazli Maula,
  • Fahim Ullah,
  • Momal Batool,
  • Mohsin Ullah,
  • Irmgard Krisai-Greilhuber,
  • Mubashar Raza

摘要

In this study, we employ integrative taxonomy and multigene phylogenetic analysis to identify and describe three novel species of the genus Clitopilus from southern Punjab, Pakistan. Detailed morphological examinations were conducted alongside advanced phylogenomic techniques, incorporating sequences from multiple genetic loci, including internal transcribed spacer (ITS), large ribosomal subunit (28S), and second largest of RNA polymerase-II (RPB2) regions. Our comprehensive morphological and phylogenetic approach allowed us to delineate distinct morphological traits and unique genetic profiles of the newly identified taxa viz. C. albominimus, C. inops, and C. pirosylvanus. The multigene phylogenetic analysis using maximum likelihood and Bayesian inference methods showed that the three new species form distinct branches, separate from any previously reported Clitopilus species, and placed them within the Clitopilus sect. Scyphoides. This research underscores the importance of integrative methods combining classical taxonomy with genomic tools to elucidate fungal biodiversity. Our findings not only expand the known diversity of Clitopilus in Pakistan but also provide valuable insights into the evolutionary relationships within the genus, paving the way for future studies on their ecological roles and conservation needs.