<p>The effect of pH on mycelial growth and ability to dissolve CaCO₃ were evaluated for 101 strains from 17 <i>Aspergillus</i> section <i>Restricti</i> species isolated from house dust in Japan. The studied strains evaluated the (1) mycelial growth on dichloran 18% glycerol or potato dextrose agar + 15% NaCl adjusted to pH 3, 5, 7, 9, and 11 and (2) ability to dissolve CaCO₃ on CaCO₃ glucose agar supplemented with 22% glycerol or 15% NaCl to adjust the water activity. Four series <i>Restricti</i> species (<i>A. conicus</i>, <i>A. domesticus</i>, <i>A. gracilis</i>, and <i>A. pseudogracilis</i>) showed optimal growth at a weakly acidic pH (pH 5) compared to other series. Two series <i>Restricti</i> species (<i>A. caesiellus</i> and <i>A. restrictus</i>) and all series <i>Penicillioides</i> species showed greater mycelial growth at pH 11 than the other series. Three series, excluding series <i>Halophilici</i>, dissolved CaCO₃. CaCO₃ dissolution abilities of six series <i>Restricti</i> species (<i>A. conicus</i>, <i>A. domesticus</i>, <i>A. gracilis</i>, <i>A. pseudogracilis</i>, <i>A. restrictus</i>, and <i>A. villosus</i>) and <i>A. vitricola</i> (series <i>Vitricolarum</i>) were stronger than those of species in other series. These results indicate that the species with different physiological characteristics are distributed in house dust. The species that have been detected in marine and limestone substrates by previous studies were found to have pH characteristics and CaCO₃ dissolution abilities suitable for survival in their respective environments.</p>