<p>Members of the fungal kingdom exhibit remarkable biodiversity and potential for use in biotechnology and industry. Therefore, their long-term preservation is very important and it is necessary to continuously develop new methods and improve existing ones. Currently, cryopreservation appears to be the best available technique for the preservation of fungi. This study summarises the latest trends and advances in fungal cryopreservation over the last eight years, i.e. findings from scientific publications from 2017 to 2024 dealing with cryopreservation of both basidiomycetes and non-basidiomycete fungi. The work analysed the organisms studied and the cryopreservation method used, focusing on the use of new procedures and modifications of existing methods; summarised the conditions for successful cryopreservation, such as cryopreservation time and temperature, as well as the substrate and cryoprotectant used.</p>