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<p>The genus <i>Pythium</i> sensu lato has been investigated mainly for agricultural pathogens, but there has been little research on this genus from non-agricultural environments. The diversity assessment of <i>Pythium</i> in natural environments, such as forests and rivers, is important not only for environmental preservation, but also for plant disease control that prevents invasion of agricultural fields. We isolated three plutative new species belonging to the genus <i>Elongisporangium</i> during a diversity survey in Japanese forests. Two of these species are described as <i>Elongisporangium verrucosum</i> sp. nov. and <i>Elongisporangium disparispinum</i> sp. nov. based on molecular phylogenetic analysis, growth temperature response, and morphology. The third species is represented by a single isolate that failed to produce morphologically informative structures in culture. <i>Elongisporangium verrucosum</i>, isolated from forests in Gifu and Nagano forms oogonia with rounded, dome-shaped spines not observed in closely related species. Differentiation of the protoplasm into zoospores begins inside the sporangium, and young zoospores, in the process of differentiation, are extruded to form vesicles. <i>Elongisporangium disparispinum</i>, isolated from forests in Gifu, Kochi, and Kagoshima, forms oogonia with sharp conical spines, which are characterized by variation in shape and size both between and within isolates. Phylogenetic analyses using the sequences of the rDNA internal transcribed spacer region, β-tubulin, and <i>COX1</i> and <i>COX2</i> genes showed that each lineage was independent, using both single data and concatenated sequences of the four regions.</p> Graphical abstract <p></p>

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Two new species of Elongisporangium recovered from forest environments in Japan

  • 期刊论文

  • 发表日期：

    2025年3月13日

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  • Kayoko Otsubo,
  • Koji Kageyama,
  • Hayato Masuya,
  • Shogo Kato,
  • Haruhisa Suga,
  • Ayaka Hieno

摘要

The genus Pythium sensu lato has been investigated mainly for agricultural pathogens, but there has been little research on this genus from non-agricultural environments. The diversity assessment of Pythium in natural environments, such as forests and rivers, is important not only for environmental preservation, but also for plant disease control that prevents invasion of agricultural fields. We isolated three plutative new species belonging to the genus Elongisporangium during a diversity survey in Japanese forests. Two of these species are described as Elongisporangium verrucosum sp. nov. and Elongisporangium disparispinum sp. nov. based on molecular phylogenetic analysis, growth temperature response, and morphology. The third species is represented by a single isolate that failed to produce morphologically informative structures in culture. Elongisporangium verrucosum, isolated from forests in Gifu and Nagano forms oogonia with rounded, dome-shaped spines not observed in closely related species. Differentiation of the protoplasm into zoospores begins inside the sporangium, and young zoospores, in the process of differentiation, are extruded to form vesicles. Elongisporangium disparispinum, isolated from forests in Gifu, Kochi, and Kagoshima, forms oogonia with sharp conical spines, which are characterized by variation in shape and size both between and within isolates. Phylogenetic analyses using the sequences of the rDNA internal transcribed spacer region, β-tubulin, and COX1 and COX2 genes showed that each lineage was independent, using both single data and concatenated sequences of the four regions.

Graphical abstract