Scholarlink

<p>Potato spindle tuber viroid (PSTVd) poses a major threat to potato production due to its stable, non-coding RNA genome and high infectivity. In this study, we harnessed the CRISPR/Cas13a system to confer targeted resistance against PSTVd in potato. Specific CRISPR RNAs (crRNAs) were designed to target conserved left terminal and central regions of the PSTVd genome, and two CRISPR/Cas13a vectors, A and B, were constructed to target these distinct functional domains. Using the Desiree potato cultivar, seven stable transgenic lines were generated via <i>Agrobacterium-</i>mediated transformation. Phenotypic evaluation confirmed that transgene expression did not affect normal plant growth. Quantitative assessment of PSTVd accumulation after mechanical friction inoculation showed that all transgenic lines exhibited significantly reduced viroid levels relative to wild-type controls. This reduction was observed in leaves sampled from 10 to 40 days post-inoculation and in tubers analyzed 30 days after harvest. These findings demonstrate that CRISPR/Cas13a efficiently and specifically suppresses PSTVd accumulation, establishing a durable strategy for engineering viroid-resistant potato. This study provides a successful example for applying RNA-targeting CRISPR technologies to control viroid infections in potato and advance breeding for viroid resistance in potato.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

CRISPR/Cas13a-mediated Resistance Effectively Suppresses Potato Spindle Tuber Viroid Accumulation in Transgenic Potato

  • 期刊论文

  • 发表日期：

    2026年6月29日

查看全文
  • Binyan Xu,
  • Siyu Zeng,
  • Ruhao Chen,
  • Zhiguo Fang,
  • Saiful Islam,
  • Ziyang Zhong,
  • Tao Yu,
  • Jie Zheng,
  • Xiaohui Zhan,
  • Botao Song,
  • Bihua Nie

摘要

Potato spindle tuber viroid (PSTVd) poses a major threat to potato production due to its stable, non-coding RNA genome and high infectivity. In this study, we harnessed the CRISPR/Cas13a system to confer targeted resistance against PSTVd in potato. Specific CRISPR RNAs (crRNAs) were designed to target conserved left terminal and central regions of the PSTVd genome, and two CRISPR/Cas13a vectors, A and B, were constructed to target these distinct functional domains. Using the Desiree potato cultivar, seven stable transgenic lines were generated via Agrobacterium-mediated transformation. Phenotypic evaluation confirmed that transgene expression did not affect normal plant growth. Quantitative assessment of PSTVd accumulation after mechanical friction inoculation showed that all transgenic lines exhibited significantly reduced viroid levels relative to wild-type controls. This reduction was observed in leaves sampled from 10 to 40 days post-inoculation and in tubers analyzed 30 days after harvest. These findings demonstrate that CRISPR/Cas13a efficiently and specifically suppresses PSTVd accumulation, establishing a durable strategy for engineering viroid-resistant potato. This study provides a successful example for applying RNA-targeting CRISPR technologies to control viroid infections in potato and advance breeding for viroid resistance in potato.