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<p>As in all plants, abiotic stress causes significant yield losses in the potato plant. Although the mitogen-activated protein kinase (MAPK) signalling network, which is evolutionarily conserved and has a crucial place in the abiotic stress response mechanism, has been studied in many plants, there are limited studies on the MAPK signalling network in potatoes under abiotic stress conditions. In this study, (1) the differential gene expressions of potato MAPK genes (StMAPKs), (2) their interactions with microRNAs (miRNAs), and (3) the interactions of StMAPK genes and proteins with transcription factor (TF) genes and proteins were investigated in silico. According to the results, 72 potato MAPK genes exhibited differential expression profiles under various environmental conditions. The 10 potato MAPK transcripts were up- or down-regulated by 11 miRNAs (<i>stu</i>-miR166a-5p, <i>stu</i>-miR166d-5p, <i>stu</i>-miR171d-3p, <i>stu</i>-miR172c-3p, <i>stu</i>-miR319-3p, <i>stu</i>-miR390-5p, <i>stu</i>-miR393-3p, <i>stu</i>-miR482d-3p, <i>stu</i>-miR827-5p, <i>stu</i>-miR6149-3p, <i>stu</i>-miR8035) at the post-transcriptional stage under abiotic stress conditions. Besides, some probable MAPK signal networks in a potato’s response to heat, drought, and high salinity were predicted. The proteins of StMAPKKK4-1/2, StMAPKKK16-1/2/4/5/6, StMAPKKK21-1/2, StRaf1, StMPKK2, StMPKK3-2, StMPKK5, StMPKK9, StMPK1, StMPK3-1, StMPK3-2, StMPK4-1, StMPK9-2, StMPK13, StMPK16-2, and StMPK19-4 had an active role in the signal transduction network of the abiotic stress response mechanism in potato. These MAPK proteins in the network mediated the phosphorylation of 11 TF proteins (StMYB-related (PGSC0003DMP400020008), StEIL (PGSC0003DMP4000(10490)/(47825)/(10488)/(35838)), StHSF (PGSC0003DMP400030290), StWRKY (PGSC0003DMP4000(33713)/(00156)/(21798)), StbHLH (PGSC0003DMP400016023), and StbZIP (PGSC0003DMP400036126)) in potato’s response to heat, drought, and high salinity. The results of the study on the integrative MAPK signal transduction network of potato regarding the abiotic stress response will contribute to future physiological, biochemical, and molecular studies to understand the network.</p>

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Mitogen-Activated Protein Kinase Network in Response to Abiotic Stress Factors in Potato (Solanum tuberosum L.)

  • 期刊论文

  • 发表日期：

    2026年3月2日

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  • Seher Yerlikaya,
  • Ufuk Demirel

摘要

As in all plants, abiotic stress causes significant yield losses in the potato plant. Although the mitogen-activated protein kinase (MAPK) signalling network, which is evolutionarily conserved and has a crucial place in the abiotic stress response mechanism, has been studied in many plants, there are limited studies on the MAPK signalling network in potatoes under abiotic stress conditions. In this study, (1) the differential gene expressions of potato MAPK genes (StMAPKs), (2) their interactions with microRNAs (miRNAs), and (3) the interactions of StMAPK genes and proteins with transcription factor (TF) genes and proteins were investigated in silico. According to the results, 72 potato MAPK genes exhibited differential expression profiles under various environmental conditions. The 10 potato MAPK transcripts were up- or down-regulated by 11 miRNAs (stu-miR166a-5p, stu-miR166d-5p, stu-miR171d-3p, stu-miR172c-3p, stu-miR319-3p, stu-miR390-5p, stu-miR393-3p, stu-miR482d-3p, stu-miR827-5p, stu-miR6149-3p, stu-miR8035) at the post-transcriptional stage under abiotic stress conditions. Besides, some probable MAPK signal networks in a potato’s response to heat, drought, and high salinity were predicted. The proteins of StMAPKKK4-1/2, StMAPKKK16-1/2/4/5/6, StMAPKKK21-1/2, StRaf1, StMPKK2, StMPKK3-2, StMPKK5, StMPKK9, StMPK1, StMPK3-1, StMPK3-2, StMPK4-1, StMPK9-2, StMPK13, StMPK16-2, and StMPK19-4 had an active role in the signal transduction network of the abiotic stress response mechanism in potato. These MAPK proteins in the network mediated the phosphorylation of 11 TF proteins (StMYB-related (PGSC0003DMP400020008), StEIL (PGSC0003DMP4000(10490)/(47825)/(10488)/(35838)), StHSF (PGSC0003DMP400030290), StWRKY (PGSC0003DMP4000(33713)/(00156)/(21798)), StbHLH (PGSC0003DMP400016023), and StbZIP (PGSC0003DMP400036126)) in potato’s response to heat, drought, and high salinity. The results of the study on the integrative MAPK signal transduction network of potato regarding the abiotic stress response will contribute to future physiological, biochemical, and molecular studies to understand the network.