Extraction Kinetics, Thermodynamics, Biological Characteristics of Polysaccharide From Nostoc Commune Vauch by Hot Water Assisted Extraction
摘要
Dynamics model for polysaccharide (HNCVP) extraction process from Nostoc commune Vauch was established. The process was fitted with the second Fick’s law, and exhibited a spontaneous and exothermic process. A maximal HNCVP yield was obtained under temperature of 363.15 K, extraction time of 150 min and solid-liquid ratio of 1:50 g/mL. The apparent viscosity of HNCVP affected significantly by pH and the type and intensity of ions, and increased significantly with the increase of Na+ and K+ concentration, while significant decreased after the addition of Ca2+ and Zn2+. In addition, HNCVP can inhibit the proliferation of Bovine Viral Diarrhea Virus (BVDV) by interference with 5’ UTR gene synthesis, and protection cells growth by regulating the relatively expression of IRF-1 and IRF-3 in Madin-Darby bovine kidney cells, where interferon maybe activated for virus elimination and defense. These results presented the potential of HNCVP as a functional additive in the food and pharmaceutical industries.