Pu’er Tea Isolated Paenibacillus yunnanensis and Bacillus camelliae Affect the Development of Streptococcus Mutans Biofilm Through the Co-aggregation
摘要
As a predominant etiological agent of dental caries, Streptococcus mutans plays a pivotal role in the development of dental plaque. Given that the fermentative bacteria from Pu’er tea exert inhibitory effects on Streptococcus mutans, in this study, we examined the co-aggregation interactions between nine strains of Pu’er tea fermentative bacteria and three strains of Streptococcus mutans. Notably, Bacillus camelliae 7578-1 T and Paenibacillus yunnanensis YN2T demonstrated robust co-aggregation capabilities. Treatments with sugars, heat, proteases, and amino acids, revealed that protein and saccharide molecules on bacterial surfaces significantly contribute to intergeneric co-aggregation. Importantly, strains 7578-1 T and YN2T reduced the biofilm biomass and culturable cell counts of Streptococcus mutans, indicating their potential for maintaining oral health. Furthermore, hydrophobic interactions, disulfide bonding, and hydrogen bonding were observed in dual-species biofilms. This research elucidates the functional significance of Pu’er tea fermentative bacteria in regulating oral microbial communities and lays a groundwork for further investigation into their potential utility in the prevention of dental caries.