Scholarlink

<p>Despite the widespread use of ethylenediamine (EDA) as a surface modifier, linking agent, or dopant in complex sensing systems, its potential to function as a stand-alone recognition layer for surface plasmon resonance (SPR) detection remains unexplored. Here, EDA, a small bifunctional molecule with two reactive amine groups, is demonstrated for the first time as an active SPR interface, offering a simple approach to detect methylparaben (MP), a widely used preservative and an emerging endocrine-disrupting compound of growing concern. When EDA was introduced onto gold thin film, the resulting SPR response showed a clear rise in effective refractive index from 1.450 to 1.548 across the 0 to 1 µM of MP. This trend produced a sensitivity of 0.00299° M<sup>-1</sup> with R<sup>2</sup> at 0.94271, while Langmuir–Freundlich modeling indicated a strong binding interaction with an affinity constant of 1.93608 × 10<sup>–11</sup> M<sup>-1</sup> with R<sup>2</sup> of 0.95283. To further clarify the interaction between EDA and MP, Fourier-transform infrared spectroscopy was employed to examine changes in the chemical environment of the modified surface. The spectra showed a shift in the N–H vibrational region at 3282.33&#xa0;cm<sup>-1</sup> together with the appearance of new C–O features at 1108.89&#xa0;cm<sup>-1</sup> and 106.30&#xa0;cm<sup>-1</sup>. Complementary morphological evidence was obtained through atomic force microscopy, which revealed that the initially uneven EDA surface, displaying a root-mean-square (RMS) roughness of 0.782&#xa0;nm, transformed into a film with finer and more uniformly distributed features after interaction with MP, resulting in RMS roughness of 0.871&#xa0;nm.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Performance Evaluation of Ethylenediamine-Modified Surface Plasmon Resonance Platforms for Methylparaben Detection

  • 期刊论文

  • 发表日期：

    2026年3月1日

查看全文
  • Nurul Illya Muhamad Fauzi,
  • Yap Wing Fen,
  • Hazwani Suhaila Hashim,
  • Muhammad Fahmi Anuar,
  • Faten Bashar Kamal Eddin,
  • Nur Aqilah Kamaruzzaman,
  • Nor Afiqah Nor Asri,
  • Muhammad Amir Zakwan Mohd Zailani,
  • Mazliana Ahmad Kamarudin,
  • Nurul Huda Osman,
  • Josephine Ying Chyi Liew

摘要

Despite the widespread use of ethylenediamine (EDA) as a surface modifier, linking agent, or dopant in complex sensing systems, its potential to function as a stand-alone recognition layer for surface plasmon resonance (SPR) detection remains unexplored. Here, EDA, a small bifunctional molecule with two reactive amine groups, is demonstrated for the first time as an active SPR interface, offering a simple approach to detect methylparaben (MP), a widely used preservative and an emerging endocrine-disrupting compound of growing concern. When EDA was introduced onto gold thin film, the resulting SPR response showed a clear rise in effective refractive index from 1.450 to 1.548 across the 0 to 1 µM of MP. This trend produced a sensitivity of 0.00299° M-1 with R2 at 0.94271, while Langmuir–Freundlich modeling indicated a strong binding interaction with an affinity constant of 1.93608 × 10–11 M-1 with R2 of 0.95283. To further clarify the interaction between EDA and MP, Fourier-transform infrared spectroscopy was employed to examine changes in the chemical environment of the modified surface. The spectra showed a shift in the N–H vibrational region at 3282.33 cm-1 together with the appearance of new C–O features at 1108.89 cm-1 and 106.30 cm-1. Complementary morphological evidence was obtained through atomic force microscopy, which revealed that the initially uneven EDA surface, displaying a root-mean-square (RMS) roughness of 0.782 nm, transformed into a film with finer and more uniformly distributed features after interaction with MP, resulting in RMS roughness of 0.871 nm.