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<p>In this article, an early-stage detection of detrimental <i>E. coli</i> bacteria using an SPR sensor in the NIR region (at wavelength of 1000&#xa0;nm) has been discussed. In this work, the sensor structure consists of CaF<sub>2</sub>/Ag/TiO<sub>2</sub>/poly-<span>l</span>-lysine/sensing element. The proposed structure consists of TiO<sub>2</sub> as a dielectric layer which improves plasmon excitation and enhances the performance of the SPR sensor. The thickness of the Ag layer (<i>d</i><sub><i>M</i></sub>), TiO<sub>2</sub> layer (<i>d</i><sub><i>A</i></sub>) and finally wavelength is varied while keeping protein layer thickness fixed at 5&#xa0;nm. To obtain the maximum FOM value of 9800 RIU<sup>−1</sup>, <i>d</i><sub><i>M</i></sub> at 47.3&#xa0;nm corresponding to <i>d</i><sub><i>A</i></sub> = 4.6&#xa0;nm under ORD (optimum radiation damping), consistently, the power loss ratio (PLR) is high as 1.89 with sensitivity of 196.00&#xa0;deg/RIU, FWHM of 0.0200° and field enhancement factor (FEF) of 1.03. The computed combined performance factor (CPF) is 19,001.34 μm<sup>4</sup>/RIU which is novel simulated considering the proposed structure.</p>

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Optical Biosensing and Simultaneous Detection of the Food-borne Bacterial Pathogen (Escherichia coli) Using an SPR Sensor Embedded in an Ag/TiO2/Poly-l-lysine Heterostructure Under ORD Conditions

  • 期刊论文

  • 发表日期：

    2025年8月6日

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  • Anuradha Mishra,
  • Sudhir Shukla,
  • Adarsh Chandra Mishra,
  • D. K. Dwivedi,
  • Pooja Lohia,
  • R. K. Yadav

摘要

In this article, an early-stage detection of detrimental E. coli bacteria using an SPR sensor in the NIR region (at wavelength of 1000 nm) has been discussed. In this work, the sensor structure consists of CaF2/Ag/TiO2/poly-l-lysine/sensing element. The proposed structure consists of TiO2 as a dielectric layer which improves plasmon excitation and enhances the performance of the SPR sensor. The thickness of the Ag layer (dM), TiO2 layer (dA) and finally wavelength is varied while keeping protein layer thickness fixed at 5 nm. To obtain the maximum FOM value of 9800 RIU−1, dM at 47.3 nm corresponding to dA = 4.6 nm under ORD (optimum radiation damping), consistently, the power loss ratio (PLR) is high as 1.89 with sensitivity of 196.00 deg/RIU, FWHM of 0.0200° and field enhancement factor (FEF) of 1.03. The computed combined performance factor (CPF) is 19,001.34 μm4/RIU which is novel simulated considering the proposed structure.