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<p><i>Clostridium tetani</i> produce a potent neuro toxoid as tetanus toxoid, which elicits spastic paralysis in humans and warm-blooded animals. Tetanus recognition is crucial; however, existing diagnostic methods have notable limitations. Recently, the localized surface plasmon resonance (LSPR) nano-bio-probe has been established as a promising technique for detecting <i>Clostridium tetani</i> toxoid. The designed nanobioprobe utilizes covalent conjugation between monoclonal anti-tetanus IgG antibodies and gold nanoparticles (GNPs). The dynamic light scattering (DLS) technique was employed to verify successful conjugation. Subsequently, the sensitivity of the LSPR was assessed as a function of tetanus toxoid concentration using a colorimetric assay. The results demonstrated that the LSPR band of functionalized GNPs exhibited a redshift from 530 to 563&#xa0;nm in the presence of 1&#xa0;ng of tetanus toxoid, outperforming the ELISA method. This study presents a rapid, sensitive, and selective approach for tetanus toxoid detection in clinical samples.</p>

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Antibody-Functionalized Gold Nanoparticles for Sensitive Detection of Tetanus Toxoid

  • 期刊论文

  • 发表日期：

    2025年7月23日

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  • Pardis Saeedi,
  • Hamideh Rouhani Nejad

摘要

Clostridium tetani produce a potent neuro toxoid as tetanus toxoid, which elicits spastic paralysis in humans and warm-blooded animals. Tetanus recognition is crucial; however, existing diagnostic methods have notable limitations. Recently, the localized surface plasmon resonance (LSPR) nano-bio-probe has been established as a promising technique for detecting Clostridium tetani toxoid. The designed nanobioprobe utilizes covalent conjugation between monoclonal anti-tetanus IgG antibodies and gold nanoparticles (GNPs). The dynamic light scattering (DLS) technique was employed to verify successful conjugation. Subsequently, the sensitivity of the LSPR was assessed as a function of tetanus toxoid concentration using a colorimetric assay. The results demonstrated that the LSPR band of functionalized GNPs exhibited a redshift from 530 to 563 nm in the presence of 1 ng of tetanus toxoid, outperforming the ELISA method. This study presents a rapid, sensitive, and selective approach for tetanus toxoid detection in clinical samples.