<p><i>Lactobacillus acidophilus</i> (LB) can produce high-purity nanoparticles; it is a top-down biological method based on the principle of dividing metal ion bulk precursors into metal atoms; this biosynthesis method was used in this work to synthesize CoCrCuFeNi high-entropy alloy. The characteristics of the synthesized nanoparticles were examined using XRD, SEM, and FTIR analysis. Subsequently, their antibacterial and antibiofilm activities were tested against a Gram-positive bacteria <i>Staphylococcus aureus</i> (<i>S. aureus</i>) and a Gram-negative bacteria <i>Escherichia coli</i> (<i>E. coli</i>) that were isolated from the urinary tract infection. The results indicated that CoCrCuFeNi HEA NPs produced via the LB, along with CoCrCuFeNi HEA NPs + LB supernatant, displayed antibacterial inhibition zones measuring 35&#xa0;mm against <i>E. coli</i> and 25&#xa0;mm against <i>S. aureus</i>. On the other hand, the antibiofilm effectiveness of the CoCrCuFeNi HEA NPs + LB on a catheter was determined to surpass 45% against <i>E. coli</i>.</p>

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Characterization and Antibacterial Properties of CoCrCuFeNi High-Entropy Alloy Nanoparticles Synthesized by Lactobacillus acidophilus

  • Nazar Khalaf Mahan,
  • Mohammed Faraj Al-Marjani,
  • Ganesan Krishnan,
  • Mohammed A. Salman

摘要

Lactobacillus acidophilus (LB) can produce high-purity nanoparticles; it is a top-down biological method based on the principle of dividing metal ion bulk precursors into metal atoms; this biosynthesis method was used in this work to synthesize CoCrCuFeNi high-entropy alloy. The characteristics of the synthesized nanoparticles were examined using XRD, SEM, and FTIR analysis. Subsequently, their antibacterial and antibiofilm activities were tested against a Gram-positive bacteria Staphylococcus aureus (S. aureus) and a Gram-negative bacteria Escherichia coli (E. coli) that were isolated from the urinary tract infection. The results indicated that CoCrCuFeNi HEA NPs produced via the LB, along with CoCrCuFeNi HEA NPs + LB supernatant, displayed antibacterial inhibition zones measuring 35 mm against E. coli and 25 mm against S. aureus. On the other hand, the antibiofilm effectiveness of the CoCrCuFeNi HEA NPs + LB on a catheter was determined to surpass 45% against E. coli.