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Nickel Nanocluster as a Fluorescent Probe for the Non-enzymatic Detection of Cardiac Troponin I

  • Ali Ibrahim Shkhair,
  • Anju S. Madanan,
  • Susan Varghese,
  • Merin K. Abraham,
  • Geneva Indongo,
  • Greeshma Rajeevan,
  • B. K. Arathy,
  • Sara Muneer Abbas,
  • Sony George

摘要

Troponin I, as a cardiac-specific biomarker, is a very valuable tool in the identification of acute coronary syndromes. In this work, an economical technique for easy and sensitive diagnosis of cardiac troponin I (cTnI) was developed nanoprobe on the basis of graphene oxide quenching of the fluorescence of bovine serum albumin–capped fluorescent nickel (BSA@NiNCs) nanoclusters. The developed nanoprobe nickel nanocluster exhibited superparamagnetism. The nanoprobe showed a fluorescence turn-on behavior toward incremental addition of in cTnI, with a detection limit of 0.012 ng/mL. We successfully used the proposed sensing technique for cTnI sensing in serum samples.