An Experimental Approach for the Detection and Analysis of Ascorbic Acid Using Optical Fiber-Based Biosensor with LSPR

The presented article is an exploratory proceed toward the diagnosis of ascorbic acid (AA) using the localized surface plasmon resonance (LSPR) methodology in conjunction with a single-mode-step (SMS) optical fiber structure. This fabricated biosensor presents simplicity, cost-effectiveness, and high sensitivity. To fabricate the suggested biosensor, a specified length of optical fiber is immersed within a solution restrain AA. The outer surface of the fiber is coated by gold nanoparticles, which generate localized surface plasmons when in contact with AA particles. The LSPR phenomenon results in a shift in the resonant wavelength of transmitted light, corresponding to AA absorptions. Laboratory appraisals have been conducted to optimize critical parameters, together with the length of the fiber, nanoparticle size, concentration, including sensing mechanisms. These appraisals involved AA concentrations and give a linear relationship in between wavelength shift as well as AA absorptions. This fabricated sensor has shown a good stability and consistency. Additionally, this biosensor holds significant potential for applications in the fields of food inspection, clinical investigations, including pharmaceutical research. This laboratory work involves the development of LSPR biosensor based on optical fiber structures, enhancing investigative capabilities.