<p>This research examines the process of appropriation and use of the maritime environment of the first port of the Malvinas/Falkland Islands, spanning from its foundation in 1764 to its abandonment as a port city and subsequent conversion into a space for livestock exploitation in 1845. From a historical archaeology perspective, this study identifies, characterizes and analyzes the key components of the settlement’s Maritime Cultural Landscape (MCL) focusing on the region’s navigability and connectivity at different scales. The investigation integrates documentary analysis with remote sensing technologies and a Geographic Information System for spatial and geographic analysis, offering a new approach to the study of historical maritime networks and navigation in the South Atlantic. The findings provide insights into historical maritime routes, seasonal navigation patterns, and the environmental and anthropogenic factors that shape the MCL of the Malvinas/Falklands. Beyond deepening the historical and cultural understanding of the region from a nautical and MCL perspective, this approach also provides a valuable tool for evaluating the site’s archaeological potential. By enabling the identification of high-interest zones—such as anchorages and port structures—it facilitates the planning of future fieldwork.</p>