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<p>In order to mitigate the coupling effect of bacterial organic matter and gravity on the heterogeneous distribution of calcium carbonate during MICP process, a bacterial organic matter control method for freeze–thaw resistance of MICP bio-cement was proposed. A series of laboratory tests were conducted on MICP bio-cement with varying bacterial organic matter contents and freeze–thaw cycles. A ternary precipitation structure induced by bacterial organic matter and gravity was identified, and the freeze–thaw resistance mechanism was analyzed. The main conclusions are as follows: (1) Adjusting the bacterial organic matter content during the MICP process to optimize freeze–thaw resistance performance is feasible, attributed to improved physicomechanical properties. Shear strength increased by 1.3 times (from 1386 to 1803&#xa0;kPa) and P-wave velocity by 1.1 times (from 3020&#xa0;m/s to 3439&#xa0;m/s) with bacterial organic matter content increasing from 100 to 300%. (2) The ternary precipitation structure consists of small crystal layer, big crystal layer, and interlaminar gap. Higher organic matter content resulted in smaller crystals in both layers and a larger interlaminar gap. (3) The interlaminar gap is the main weak layer during freeze–thaw cycles. Sample S1 with 200% bacterial organic matter content exhibited better freeze–thaw resistance than the control sample after 10 freeze–thaw cycles, with a 15% increase in shear strength (from 1270 to 1463&#xa0;kPa).</p>

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Bacterial organic matter-based control method for freeze–thaw resistance improvement of MICP bio-cement

  • 期刊论文

  • 发表日期：

    2025年9月1日

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  • Yu-Jia Liu,
  • Xiao-Hua Pan,
  • Chao-Sheng Tang,
  • Rui Wang,
  • Zhi-Hao Dong,
  • Qi-Chen Dai,
  • Guang-Hui Lei,
  • Shao-Dan Wang

摘要

In order to mitigate the coupling effect of bacterial organic matter and gravity on the heterogeneous distribution of calcium carbonate during MICP process, a bacterial organic matter control method for freeze–thaw resistance of MICP bio-cement was proposed. A series of laboratory tests were conducted on MICP bio-cement with varying bacterial organic matter contents and freeze–thaw cycles. A ternary precipitation structure induced by bacterial organic matter and gravity was identified, and the freeze–thaw resistance mechanism was analyzed. The main conclusions are as follows: (1) Adjusting the bacterial organic matter content during the MICP process to optimize freeze–thaw resistance performance is feasible, attributed to improved physicomechanical properties. Shear strength increased by 1.3 times (from 1386 to 1803 kPa) and P-wave velocity by 1.1 times (from 3020 m/s to 3439 m/s) with bacterial organic matter content increasing from 100 to 300%. (2) The ternary precipitation structure consists of small crystal layer, big crystal layer, and interlaminar gap. Higher organic matter content resulted in smaller crystals in both layers and a larger interlaminar gap. (3) The interlaminar gap is the main weak layer during freeze–thaw cycles. Sample S1 with 200% bacterial organic matter content exhibited better freeze–thaw resistance than the control sample after 10 freeze–thaw cycles, with a 15% increase in shear strength (from 1270 to 1463 kPa).