Bacterial population dynamics and biosafety assessment in a closed Bioregenerative Life Support System during the “Lunar Palace 365” experiment
摘要
Our study investigates the dynamic variation of bacterial populations within the Bioregenerative Life Support System (BLSS), a critical component for sustainable habitats in extraterrestrial environments supporting human survival. We utilized the ground-based BLSS test platform, referred to as “Lunar Palace 1” (LP1), to examine bacterial dynamics in an ecosystem integrating plant production, human cultivation, and equipment maintenance. Our findings reveal distinct bacterial compositions among crew members and functional areas within the LP1 system while preserving temporal stability across the 370-day experiment. Importantly, we observed a low abundance of potential pathogens, minimal antibiotic resistance, and negligible impact of the isolated bacterial strains on equipment materials, suggesting favorable biosafety attributed to plant integration. Our work enhances the understanding of microbial communities in BLSS and underscores the importance of incorporating green plants to bolster environmental biosafety and sustainability in long-term space exploration missions.