<p>The deliberate and precise fabrication of supported catalysts with well-defined structures has been a long-standing pursuit. Among the large number of supports for anchoring noble metal species, layered double hydroxides (LDHs) stand out as a class of ideal supports for dispersing noble metal species due to their uniform distribution of metal sites and charge centers. However, a prerequisite for the investigation of noble metals-loaded LDHs is the construction of well-defined active sites and precisely controlled sizes of noble metal species on LDH surfaces. Based on research advances made over the past decade, we provide a detailed discussion on the fine regulation of noble metal species, including single atoms, nanoclusters, nanoparticles, and multi-site catalysts on LDHs. Furthermore, we focus on uncovering three critical aspects of noble metal-loaded LDHs: the precise location, the atomic configurations, and the interaction with the LDHs. Building upon these well-defined structures, we further clarify the structure-activity relationships of the developed materials in reactions such as hydrazine decomposition, biomass conversion, C-H bond activation, and water splitting, etc. Finally, the challenges and future directions are outlined to offer valuable insights for advancing high-activity noble metal-loaded LDHs.</p>