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<p>Effectively managing bone defects presents significant clinical challenges, where bone marrow mesenchymal stem cells (BMSCs) offer promising potential for bone regeneration. However, delivering BMSCs effectively requires suitable biomaterials. Herein, we introduce, <b>Pep1</b>, a calcium-responsive peptide designed for delivering BMSCs for femur regeneration. <b>Pep1</b> self-assembles to form a pericellular hydrogel in the presence of calcium ions, forming a nanofiber-rich network conductive to BMSCs encapsulation. Important, <b>Pep1</b> hydrogel supports BMSC activity in 3D cell culture without compromise. <i>In vitro</i> and <i>in vivo</i> studies demonstrate excellent biocompatibility of <b>Pep1</b> hydrogel. Osteogenic differentiation and q-PCR assays reveal that <b>Pep1</b> enhances osteogenic differentiation and upregulates bone-related genes expression. Furthermore, <i>In vivo</i> experiments confirm that <b>Pep1</b> hydrogel encapsulating BMSCs significantly improves femur defect repair. This study underscores <b>Pep1</b> as a promising biomaterial for calcium-responsive bone tissue regeneration.</p>

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Calcium-responsible injectable peptide hydrogel encapsulating BMSCs for promoting femur regeneration

  • 期刊论文

  • 发表日期：

    2025年1月7日

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  • Xia Wu,
  • Tingfen Deng,
  • Wenbing Liu,
  • Hong Han,
  • Wei Xie,
  • Wenjie Wang,
  • Dan Yuan,
  • Lisha Zha,
  • Junfeng Shi,
  • Xiaoyun Pan

摘要

Effectively managing bone defects presents significant clinical challenges, where bone marrow mesenchymal stem cells (BMSCs) offer promising potential for bone regeneration. However, delivering BMSCs effectively requires suitable biomaterials. Herein, we introduce, Pep1, a calcium-responsive peptide designed for delivering BMSCs for femur regeneration. Pep1 self-assembles to form a pericellular hydrogel in the presence of calcium ions, forming a nanofiber-rich network conductive to BMSCs encapsulation. Important, Pep1 hydrogel supports BMSC activity in 3D cell culture without compromise. In vitro and in vivo studies demonstrate excellent biocompatibility of Pep1 hydrogel. Osteogenic differentiation and q-PCR assays reveal that Pep1 enhances osteogenic differentiation and upregulates bone-related genes expression. Furthermore, In vivo experiments confirm that Pep1 hydrogel encapsulating BMSCs significantly improves femur defect repair. This study underscores Pep1 as a promising biomaterial for calcium-responsive bone tissue regeneration.