Scholarlink

Purpose <p>New LSD analogs are a newly emerging and basically under-researched group of new psychoactive substances (NPS). The emergence of a completely new group of NPS make it difficult to monitor intoxications with these compounds. Moreover, forensic toxicological evaluation often requires comprehensive information on changes in the concentration of NPS in biological specimens over time following their collection.</p> Methods <p>In this study, 12 LSD analogs were analyzed to determine their stability in whole blood and dry blood spots (DBS). The samples were stored at room temperature (DBS), 4&#xa0;°C (DBS and whole blood), and − 20&#xa0;°C (whole blood). Analyses were performed using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry. The stability study lasted 365 days, monitoring both the degradation rate of analytes and formation of degradation products in vitro. Moreover, the effect of chemical structure on analyte stability was assessed.</p> Results <p>Results proved that the stability of analytes depended on both storage conditions and their chemical structure. Majority of compounds showed better long-term stability in DBS samples. Furthermore, <i>N</i>-deacylation of ALD-52, 1P-LSD, 1B-LSD, 1&#xa0;V-LSD, 1cP-MiPLA, and 1P-MiPLA was observed, which was more pronounced for LSD derivatives than for MiPLA derivatives.</p> Conclusions <p>Studies indicate a strong correlation between the stability of LSD analogs and both their chemical structure and storage conditions. DBS cards frequently provide equaled or enhanced stability compared to whole blood, which makes it advisable to collect biological material in both forms to help maximize analytical options in forensic and toxicological investigations.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Comparative stability assessment of novel LSD analogs in dry blood spots and blood using UHPLC-MS/MS: implications for forensic applications

  • 期刊论文

  • 发表日期：

    2026年6月14日

查看全文
  • Kaja Tusiewicz,
  • Paweł Szpot,
  • Marcin Zawadzki

摘要

Purpose

New LSD analogs are a newly emerging and basically under-researched group of new psychoactive substances (NPS). The emergence of a completely new group of NPS make it difficult to monitor intoxications with these compounds. Moreover, forensic toxicological evaluation often requires comprehensive information on changes in the concentration of NPS in biological specimens over time following their collection.

Methods

In this study, 12 LSD analogs were analyzed to determine their stability in whole blood and dry blood spots (DBS). The samples were stored at room temperature (DBS), 4 °C (DBS and whole blood), and − 20 °C (whole blood). Analyses were performed using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry. The stability study lasted 365 days, monitoring both the degradation rate of analytes and formation of degradation products in vitro. Moreover, the effect of chemical structure on analyte stability was assessed.

Results

Results proved that the stability of analytes depended on both storage conditions and their chemical structure. Majority of compounds showed better long-term stability in DBS samples. Furthermore, N-deacylation of ALD-52, 1P-LSD, 1B-LSD, 1 V-LSD, 1cP-MiPLA, and 1P-MiPLA was observed, which was more pronounced for LSD derivatives than for MiPLA derivatives.

Conclusions

Studies indicate a strong correlation between the stability of LSD analogs and both their chemical structure and storage conditions. DBS cards frequently provide equaled or enhanced stability compared to whole blood, which makes it advisable to collect biological material in both forms to help maximize analytical options in forensic and toxicological investigations.