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<p>This research aims to screen the active fraction and explore the action mechanism of <i>Ganoderma lucidum</i> to combat asthma using asthmatic mice induced by OVA. In this study, three <i>Ganoderma lucidum</i> fractions, including GLEF40, GLEF60, and GLEF100, were obtained through the separation of D101 macroporous adsorption resin. The result of the anti-asthmatic effects illustrated that GLEFs significantly reduce Th2- and Th1- cytokines, together with chemokines MIP-1α and MDC in bronchoalveolar lavage fluid (BALF) in asthmatic mice. Among the three fractions, GLEF100 demonstrated the most potent anti-inflammatory effect. Besides, GLEF100 significantly reduced IgE levels in asthmatic mice (<i>P</i> &lt; 0.001). Further analysis found that GLEF100 significantly reduced Myd88 protein levels and strongly inhibits the expression of phosphorylated ERK, phosphorylated JNK and NF-κB p65. To further explore the Anti-asthmatic active component <i>Ganoderma lucidum</i>, a total of 92 chemical components from GLE100 was annotated using the HPLC–Q-TOF–MS/MS. In conclusion, GLEF100 effectively relieving asthma symptoms by inducing balanced Th1/Th2 immunity and inhibiting TLR4/Myd88-NF-κB-MAPKs signaling pathway. These findings highlight the potential anti-asthmatic effects of GLEF 100 and lay the foundation for further identification of the anti-asthmatic active components of <i>Ganoderma lucidum</i>.</p> Graphical abstract <p></p>

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Anti-asthmatic effects and ingredient annotation of the active fraction from Ganoderma lucidum

  • 期刊论文

  • 发表日期：

    2026年3月26日

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  • Ning Li,
  • Qian He,
  • Cunwu W. Chen,
  • Qun Zhao,
  • Yanfei F. He

摘要

This research aims to screen the active fraction and explore the action mechanism of Ganoderma lucidum to combat asthma using asthmatic mice induced by OVA. In this study, three Ganoderma lucidum fractions, including GLEF40, GLEF60, and GLEF100, were obtained through the separation of D101 macroporous adsorption resin. The result of the anti-asthmatic effects illustrated that GLEFs significantly reduce Th2- and Th1- cytokines, together with chemokines MIP-1α and MDC in bronchoalveolar lavage fluid (BALF) in asthmatic mice. Among the three fractions, GLEF100 demonstrated the most potent anti-inflammatory effect. Besides, GLEF100 significantly reduced IgE levels in asthmatic mice (P < 0.001). Further analysis found that GLEF100 significantly reduced Myd88 protein levels and strongly inhibits the expression of phosphorylated ERK, phosphorylated JNK and NF-κB p65. To further explore the Anti-asthmatic active component Ganoderma lucidum, a total of 92 chemical components from GLE100 was annotated using the HPLC–Q-TOF–MS/MS. In conclusion, GLEF100 effectively relieving asthma symptoms by inducing balanced Th1/Th2 immunity and inhibiting TLR4/Myd88-NF-κB-MAPKs signaling pathway. These findings highlight the potential anti-asthmatic effects of GLEF 100 and lay the foundation for further identification of the anti-asthmatic active components of Ganoderma lucidum.

Graphical abstract