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Porpose <p>Autotrophic microorganisms, especially in paddy soil, have substantial carbon (C) fixation potential, which is strongly influenced by nutrient management practices in field. However, the impacts of different fertilizer applications on microbial CO<sub>2</sub> fixation potential in paddy fields are not clearly addressed. Therefore, this study aims to investigate the impacts of paddy soil C fixation dynamics and the mechanisms driven by autotrophic microorganisms under different long-term fertilization regimes.</p> Materials and methods <p>Soil from a lasted 30 years of fertilized paddy fields was used, consisting of the four treatments: control (CK), mineral fertilizer (NPK), a combination of rice straw and mineral fertilizer (SNPK), and a combination of 30% manure with 70% mineral fertilizer (MNPK). After 28 days of continuous labeling with 5% (v/v) <sup>13</sup>C-CO<sub>2</sub>, the <sup>13</sup>C-labeled organic carbon (<sup>13</sup>C-SOC), gene abundances (<i>cbbL</i>, <i>cbbM</i> and <i>accA</i>) and the <sup>13</sup>C-PLFA population structures of each treatment soils have been analyzed.</p> Results and discussion <p>The results showed that the SNPK treatment significantly enhanced paddy soil CO<sub>2</sub> fixation capacity. The changes in CO<sub>2</sub>-fixation capacity were more greatly attributed to the shifts in microbial community composition rather than alterations in the abundance of functional autotrophic microorganisms. Notably, random forest analysis shows <sup>13</sup>C-PLFA content of actinomycetes was the key factor influencing the amount of <sup>13</sup>C-SOC assimilations, revealing that actinomycetes exhibited higher carbon fixation efficiency compared to other microbial counterparts. Moreover, the significantly positive correlation between soil C/N ratio and <sup>13</sup>C-SOC assimilations highlights the important role of nutrient availability in C-fixation of long-term fertilized paddy soil.</p> Conclusions <p>In conclusion, this study demonstrated that long-term fertilization could modulate paddy soil carbon fixation capacity by altering the soil C/N ratio.</p>

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High C/N ratio induced by fertilizer application enhance paddy soil CO2 fixation capacity under anaerobic condition

  • 期刊论文

  • 发表日期：

    2025年4月16日

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  • Ne Wu,
  • Qiong Liu,
  • Xiaomeng Wei,
  • Zhenke Zhu,
  • Xiaobin Guo,
  • Tida Ge,
  • Jinshui Wu

摘要

Porpose

Autotrophic microorganisms, especially in paddy soil, have substantial carbon (C) fixation potential, which is strongly influenced by nutrient management practices in field. However, the impacts of different fertilizer applications on microbial CO2 fixation potential in paddy fields are not clearly addressed. Therefore, this study aims to investigate the impacts of paddy soil C fixation dynamics and the mechanisms driven by autotrophic microorganisms under different long-term fertilization regimes.

Materials and methods

Soil from a lasted 30 years of fertilized paddy fields was used, consisting of the four treatments: control (CK), mineral fertilizer (NPK), a combination of rice straw and mineral fertilizer (SNPK), and a combination of 30% manure with 70% mineral fertilizer (MNPK). After 28 days of continuous labeling with 5% (v/v) 13C-CO2, the 13C-labeled organic carbon (13C-SOC), gene abundances (cbbL, cbbM and accA) and the 13C-PLFA population structures of each treatment soils have been analyzed.

Results and discussion

The results showed that the SNPK treatment significantly enhanced paddy soil CO2 fixation capacity. The changes in CO2-fixation capacity were more greatly attributed to the shifts in microbial community composition rather than alterations in the abundance of functional autotrophic microorganisms. Notably, random forest analysis shows 13C-PLFA content of actinomycetes was the key factor influencing the amount of 13C-SOC assimilations, revealing that actinomycetes exhibited higher carbon fixation efficiency compared to other microbial counterparts. Moreover, the significantly positive correlation between soil C/N ratio and 13C-SOC assimilations highlights the important role of nutrient availability in C-fixation of long-term fertilized paddy soil.

Conclusions

In conclusion, this study demonstrated that long-term fertilization could modulate paddy soil carbon fixation capacity by altering the soil C/N ratio.