Scholarlink

<p>Age-related hearing loss (ARHL) is a common condition among older adults. It has long been established that ARHL is more prevalent, is more severe, and tends to occur earlier in men than in women. A key factor contributing to ARHL is oxidative stress, which harms the inner ear cells. Sirtuins are a family of proteins that play critical roles in cellular regulation, particularly in response to stress and aging, and have demonstrated anti-aging effects. Cocoa flavonoids, due to their antioxidant properties, have been reported to provide numerous health benefits. The present study aimed to evaluate the efficacy of cocoa in preventing oxidative damage in the cochlea of aging mice mediated by Sirtuins, as well as to determine possible differences between sexes. Female and male C57Bl/6&#xa0;J mice were randomly assigned to two study groups: standard diet and cocoa-supplemented diet. At the ages of 6, 14, and 22&#xa0;months, the animals were sacrificed, and cochleae were harvested to measure the expression of Sirtuins-1, -3, forkhead box O3 (FOXO3), p53, antioxidant enzymes, oxidative DNA damage, and levels of reactive oxidative species (ROS). Our results indicated that cocoa attenuated oxidative DNA damage, reduced ROS levels, and induced the expression of SIRT1, -3, FOXO3, and p53 in the cochlea of ​​aged mice. Furthermore, the activity of antioxidant enzymes in aged mice was restored with the cocoa diet. Sex differences were observed in several of these variables. These results indicate that cocoa protects against oxidative damage through Sirtuins. In conclusion, cocoa may have therapeutic potential against ARHL.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Sirtuins mediate the reduction of age-related oxidative damage in the cochlea under a cocoa-rich diet

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年8月20日

查看全文
  • Rosalía Fátima Heredia-Molina,
  • Juan Ignacio Riestra-Ayora,
  • Israel John Thuissard Vasallo,
  • Ricardo Sanz-Fernández,
  • Carolina Sánchez-Rodríguez

摘要

Age-related hearing loss (ARHL) is a common condition among older adults. It has long been established that ARHL is more prevalent, is more severe, and tends to occur earlier in men than in women. A key factor contributing to ARHL is oxidative stress, which harms the inner ear cells. Sirtuins are a family of proteins that play critical roles in cellular regulation, particularly in response to stress and aging, and have demonstrated anti-aging effects. Cocoa flavonoids, due to their antioxidant properties, have been reported to provide numerous health benefits. The present study aimed to evaluate the efficacy of cocoa in preventing oxidative damage in the cochlea of aging mice mediated by Sirtuins, as well as to determine possible differences between sexes. Female and male C57Bl/6 J mice were randomly assigned to two study groups: standard diet and cocoa-supplemented diet. At the ages of 6, 14, and 22 months, the animals were sacrificed, and cochleae were harvested to measure the expression of Sirtuins-1, -3, forkhead box O3 (FOXO3), p53, antioxidant enzymes, oxidative DNA damage, and levels of reactive oxidative species (ROS). Our results indicated that cocoa attenuated oxidative DNA damage, reduced ROS levels, and induced the expression of SIRT1, -3, FOXO3, and p53 in the cochlea of ​​aged mice. Furthermore, the activity of antioxidant enzymes in aged mice was restored with the cocoa diet. Sex differences were observed in several of these variables. These results indicate that cocoa protects against oxidative damage through Sirtuins. In conclusion, cocoa may have therapeutic potential against ARHL.

Graphical Abstract