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<p>The growing demand for sustainable and cost-effective surfactants has intensified interest in microbial biosurfactants, yet optimizing synergistic microbial systems using industrial waste streams remains a significant challenge. This study introduces a novel co-culture strategy combining <i>Pseudomonas aeruginosa</i> and <i>Saccharomyces cerevisiae</i> for biosurfactant production, uniquely leveraging a dual-substrate approach with waste cooking oil (WCO) and diesel fuel. This design not only valorizes two abundant industrial wastes but also enhances biosurfactant yield through substrate diversification. Under controlled fermentation, the soil-derived microbial consortium exhibited exceptional emulsification performance, achieving <i>E</i><sub>24</sub> values of 83.33 ± 0.6% with diesel fuel and 73.33 ± 0.9% with WCO, while maintaining emulsion stability (ESI ≈ 0%) under optimized conditions (pH 8, 37–50&#xa0;°C). Notably, yeast extract supplementation increased diesel-based biosurfactant yield by 22%, highlighting a practical process innovation for enhanced production. The statistical analysis of the experimental data revealed that emulsification and stability were strongly influenced by synergistic interactions among temperature, pH, organic substrate, and microbial strain, emphasizing the importance of co-optimization in multi-factor systems. The proposed co-culture system demonstrates high efficiency, scalability, and robustness, offering promising commercial applications in environmental remediation (e.g., enhanced oil recovery, soil washing), eco-friendly cleaning formulations, and agricultural adjuvants. By integrating waste valorization with process optimization, this work provides a sustainable and economically viable solution for industrial biosurfactant production.</p> Graphical abstract <p></p>

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Optimization of biosurfactant production by Pseudomonas aeruginosa and Saccharomyces cerevisiae using waste cooking oil and diesel fuel

  • 期刊论文

  • 发表日期：

    2026年5月19日

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  • Boubaker FEZZANI,
  • Rihab Hedhiri,
  • BelHassen Guessemi,
  • Mahmoud Belloumi,
  • Neila Saidi

摘要

The growing demand for sustainable and cost-effective surfactants has intensified interest in microbial biosurfactants, yet optimizing synergistic microbial systems using industrial waste streams remains a significant challenge. This study introduces a novel co-culture strategy combining Pseudomonas aeruginosa and Saccharomyces cerevisiae for biosurfactant production, uniquely leveraging a dual-substrate approach with waste cooking oil (WCO) and diesel fuel. This design not only valorizes two abundant industrial wastes but also enhances biosurfactant yield through substrate diversification. Under controlled fermentation, the soil-derived microbial consortium exhibited exceptional emulsification performance, achieving E24 values of 83.33 ± 0.6% with diesel fuel and 73.33 ± 0.9% with WCO, while maintaining emulsion stability (ESI ≈ 0%) under optimized conditions (pH 8, 37–50 °C). Notably, yeast extract supplementation increased diesel-based biosurfactant yield by 22%, highlighting a practical process innovation for enhanced production. The statistical analysis of the experimental data revealed that emulsification and stability were strongly influenced by synergistic interactions among temperature, pH, organic substrate, and microbial strain, emphasizing the importance of co-optimization in multi-factor systems. The proposed co-culture system demonstrates high efficiency, scalability, and robustness, offering promising commercial applications in environmental remediation (e.g., enhanced oil recovery, soil washing), eco-friendly cleaning formulations, and agricultural adjuvants. By integrating waste valorization with process optimization, this work provides a sustainable and economically viable solution for industrial biosurfactant production.

Graphical abstract