<p>This study presents the synthesis and characterization of rGO/CS/Ag nanozyme, an artificial enzyme with peroxidase-like activity. A complex nanozyme composed of reduced graphene oxide (rGO), silver (Ag), and chitosan (CS) is reported in this study for the first time as a catalyst material and synthetic enzyme for detecting the carbaryl pesticide. FTIR analysis confirmed the reduction of graphene oxide (GO) to reduced graphene oxide (rGO) using urea, which removes oxygen-containing functional groups while still leaving some oxygen groups intact. XRD analysis showed a reduction in the interlayer spacing of rGO after reduction, with the nanozyme displaying a uniform distribution of Ag on the rGO surface. SEM and BET analysis revealed a mesoporous structure in the rGO/CS/Ag nanozyme, with a large surface area suitable for catalytic applications. The catalytic activity was tested using a TMB-H₂O₂ system, and the results showed good peroxidase activity, with the nanozyme showing a high affinity for the TMB substrate. Furthermore, inhibition studies using the pesticide carbaryl confirmed that the nanozyme operates through a non-competitive inhibition mechanism. Carbaryl detection was successfully achieved with a low detection limit (0.0036 mM), demonstrating the potential of this nanozyme for environmental monitoring and sensor applications. This study highlights the promising catalytic and detection capabilities of the rGO/CS/Ag nanozyme in various applications, including catalysis and sensing.</p>