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<p>Soil petroleum pollution is becoming increasingly serious, and microbial degradation is an important means for petroleum bioremediation. In this study, four petroleum-contaminated soil samples were collected at a single site in Puyang, Henan, China, with different petroleum concentrations to investigate the microbial structure, functional prediction, and microbial network structure. The results showed that petroleum significantly affected the composition and structure of the bacterial community, and that both richness and diversity were negatively correlated with petroleum concentration. A high concentration of petroleum changed the relative abundance of different taxa in soil (such as <i>Salinimicrobium</i>, increasing from 0.3% to 23.4%) and enhanced the relative abundance of 19 soil functions, such as amino acid metabolism. Network analysis revealed the key bacterial groups in petroleum-contaminated soil, including <i>Salinimicrobium</i> and <i>Sphingomonas</i>. Taxonomic differences and network analysis identified <i>Salinimicrobium</i> as a potential petroleum-degrading bacterium. Subsequently, a strain named <i>Salinimicrobium</i> sp. SYJJ-1 was successfully isolated from soil contaminated with a high concentration of petroleum. It degraded 47.8% of 3.0 g·L<sup>−1</sup> petroleum in medium within 7 days. Furthermore, this strain also possessed a 61.9% degradation rate for 10.0 g·kg<sup>−1</sup> petroleum in soil within 90 days. This study provides guidance for understanding the effects of petroleum on the soil bacterial community and the targeted isolation of pollutant-degrading bacteria from soil.</p>

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Soil bacterial community analysis guides the isolation of petroleum-degrading bacteria and potential application for the bioremediation of petroleum-contaminated soil

  • 期刊论文

  • 发表日期：

    2025年10月1日

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  • Kai Huo,
  • Zhihua Sun,
  • Lili Zhao,
  • Yimin Hao,
  • Ziwei Hua,
  • Qiuhong Niu,
  • Pengfei Duan,
  • Hao Zhang

摘要

Soil petroleum pollution is becoming increasingly serious, and microbial degradation is an important means for petroleum bioremediation. In this study, four petroleum-contaminated soil samples were collected at a single site in Puyang, Henan, China, with different petroleum concentrations to investigate the microbial structure, functional prediction, and microbial network structure. The results showed that petroleum significantly affected the composition and structure of the bacterial community, and that both richness and diversity were negatively correlated with petroleum concentration. A high concentration of petroleum changed the relative abundance of different taxa in soil (such as Salinimicrobium, increasing from 0.3% to 23.4%) and enhanced the relative abundance of 19 soil functions, such as amino acid metabolism. Network analysis revealed the key bacterial groups in petroleum-contaminated soil, including Salinimicrobium and Sphingomonas. Taxonomic differences and network analysis identified Salinimicrobium as a potential petroleum-degrading bacterium. Subsequently, a strain named Salinimicrobium sp. SYJJ-1 was successfully isolated from soil contaminated with a high concentration of petroleum. It degraded 47.8% of 3.0 g·L−1 petroleum in medium within 7 days. Furthermore, this strain also possessed a 61.9% degradation rate for 10.0 g·kg−1 petroleum in soil within 90 days. This study provides guidance for understanding the effects of petroleum on the soil bacterial community and the targeted isolation of pollutant-degrading bacteria from soil.