Scholarlink

<p>The distribution of two waterborne pathogens linked to global antibiotic resistance, <i>Aeromonas caviae</i> (Acav) and <i>Pseudomonas aeruginosa</i> (Paer), was examined across a mixed-use catchment. Their emission sources and impacts on water quality were assessed. A novel quantitative PCR (qPCR) assay for Acav was developed. Pathogen tracking via qPCR was compared with microbial source tracking (qMST) of fecal contamination and with land use and physicochemical data. Acav prevalence was highest in road runoff, with counts correlating with human, ruminant, and sewer MST markers and nutrient levels. Real-time in vitro assays using Caco-2 intestinal epithelial cells demonstrated the infectious potential of these freshwater Acav cells. Paer was most abundant in brooks located in forested areas. Markers from the qPCR/qMST toolbox were used to estimate the&#xa0;relative contribution of headwaters, road runoff, and wastewater to stream outlets during rain events. Sewer outflows and first-order brook waters draining ruminant pasture fields were identified as key sources of downstream microbial contaminants. Road runoff containing Acav and forested headwaters harboring Paer further explained the observed microbial profiles. These findings revealed the streams’ poor hygienic status and potential health risks for humans and animals. The toolbox proved effective in identifying pathogen sources and assessing their influence on water quality, delivering critical insights for enhancing the management of mixed-use catchments facing increasing pressures from climate change.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Tracking Aeromonas caviae and Pseudomonas aeruginosa in a mixed-use catchment with source attribution, health hazards, and microbial mixing assessments

  • 期刊论文

  • 发表日期：

    2025年10月1日

查看全文
  • Adrien C. M. Pozzi,
  • Angélique Dominguez-Lage,
  • Baptiste Luton,
  • Laurence Marjolet,
  • Delphine Mouniée,
  • Emmanuelle Bergeron,
  • Matthieu Masson,
  • Cécile Miège,
  • Marina Coquery,
  • Pauline Dusseux,
  • Nicolas Robinet,
  • Véronica Rodriguez-Nava,
  • Emmanuelle Danty-Berger,
  • Olivier Grandjouan,
  • Mickaël Lagouy,
  • Flora Branger,
  • Wessam Galia,
  • Benoit Cournoyer

摘要

The distribution of two waterborne pathogens linked to global antibiotic resistance, Aeromonas caviae (Acav) and Pseudomonas aeruginosa (Paer), was examined across a mixed-use catchment. Their emission sources and impacts on water quality were assessed. A novel quantitative PCR (qPCR) assay for Acav was developed. Pathogen tracking via qPCR was compared with microbial source tracking (qMST) of fecal contamination and with land use and physicochemical data. Acav prevalence was highest in road runoff, with counts correlating with human, ruminant, and sewer MST markers and nutrient levels. Real-time in vitro assays using Caco-2 intestinal epithelial cells demonstrated the infectious potential of these freshwater Acav cells. Paer was most abundant in brooks located in forested areas. Markers from the qPCR/qMST toolbox were used to estimate the relative contribution of headwaters, road runoff, and wastewater to stream outlets during rain events. Sewer outflows and first-order brook waters draining ruminant pasture fields were identified as key sources of downstream microbial contaminants. Road runoff containing Acav and forested headwaters harboring Paer further explained the observed microbial profiles. These findings revealed the streams’ poor hygienic status and potential health risks for humans and animals. The toolbox proved effective in identifying pathogen sources and assessing their influence on water quality, delivering critical insights for enhancing the management of mixed-use catchments facing increasing pressures from climate change.

Graphical Abstract