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<p>The dissemination of the World Health Organization critical-priority <i>Enterobacterales</i> in natural ecosystems presents significant global health concerns. Here, we report the occurrence of metal-tolerant <i>Klebsiella pneumoniae</i> and <i>Escherichia coli</i> clones, typically linked to healthcare settings, carrying KPC-2, CTX-M-type, and/or RmtB resistance mechanisms, in wild-caught Atlantic blue crabs (<i>Callinectes sapidus</i>) from a polluted estuarine area in southeast Brazil. Thirteen crabs were collected and processed, leading to the isolation of three meropenem-resistant <i>K. pneumoniae</i> and five ceftriaxone-resistant <i>E. coli</i> isolates. Genomic analyses confirmed the presence of carbapenemases and extended-spectrum-β-lactamases (ESBLs). KPC-2-positive <i>K</i>. <i>pneumoniae</i> isolates were assigned as high-risk clones [sequence type (ST) ST11 and ST258], whereas CTX-M (-8, -14, -15)-ESBL-producing <i>E. coli</i> isolates belonged to international (ST196, ST345, and ST8319) and pandemic (ST38 and ST617) clones. The novel Tn<i>4401l</i> isoform, with 72-bp deletion between IS<i>Kpn7</i> and <i>bla</i><sub>KPC-2</sub>,&#xa0;was identified in a <i>K. pneumoniae</i> isolate. The crab-derived isolates also presented multimetal tolerance with the presence of plasmid-mediated metal tolerance operons. These findings highlight the importance of examining the genetic patterns of bacteria commonly found in humans as they expand into new environments, potentially&#xa0;posing significant health risks.</p>

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Multimetal tolerance in high-risk clones of KPC-2- and CTX-M-producing Enterobacterales isolated from Atlantic blue crabs in an anthropogenically contaminated estuarine environment

  • 期刊论文

  • 发表日期：

    2025年9月1日

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  • Fábio Parra Sellera,
  • Daniel Farias Marinho Monte,
  • João Pedro Rueda Furlan,
  • Andrey Guimarães Sacramento,
  • Jesus Giovani Mamani Pariona,
  • Maria Espinoza-Muñoz,
  • Edson Aparecido da Silva,
  • Milton Ricardo Azedo,
  • Marcelo Pires Nogueira Carvalho,
  • Mariza Landgraf,
  • Nilton Lincopan

摘要

The dissemination of the World Health Organization critical-priority Enterobacterales in natural ecosystems presents significant global health concerns. Here, we report the occurrence of metal-tolerant Klebsiella pneumoniae and Escherichia coli clones, typically linked to healthcare settings, carrying KPC-2, CTX-M-type, and/or RmtB resistance mechanisms, in wild-caught Atlantic blue crabs (Callinectes sapidus) from a polluted estuarine area in southeast Brazil. Thirteen crabs were collected and processed, leading to the isolation of three meropenem-resistant K. pneumoniae and five ceftriaxone-resistant E. coli isolates. Genomic analyses confirmed the presence of carbapenemases and extended-spectrum-β-lactamases (ESBLs). KPC-2-positive K. pneumoniae isolates were assigned as high-risk clones [sequence type (ST) ST11 and ST258], whereas CTX-M (-8, -14, -15)-ESBL-producing E. coli isolates belonged to international (ST196, ST345, and ST8319) and pandemic (ST38 and ST617) clones. The novel Tn4401l isoform, with 72-bp deletion between ISKpn7 and blaKPC-2, was identified in a K. pneumoniae isolate. The crab-derived isolates also presented multimetal tolerance with the presence of plasmid-mediated metal tolerance operons. These findings highlight the importance of examining the genetic patterns of bacteria commonly found in humans as they expand into new environments, potentially posing significant health risks.