Evaluation of morphological and cytotoxic effects of minoxidil on Phaseolus vulgaris L. as a plant model

The increasing presence of drugs in the environment has triggered a pollution crisis on a global scale, generating concern about their ecotoxicological effects on ecosystems. In this context, minoxidil, a vasodilator drug widely used in the treatment of androgenic alopecia, has been scarcely investigated in plant systems. Therefore, this study evaluated the morphological and cytotoxic effects of minoxidil on Phaseolus vulgaris L as a bioindicator. P. vulgaris seeds were exposed to different concentrations of minoxidil (0.0, 0.25, 0.5, 1, 1.25, 2.5 and 5 mg/L) and parameters such as germination, root growth, mitotic index and frequency of chromosomal abnormalities were assessed. The results showed a significant inhibition of germination (75–76%) and root growth (52.6–55.3%) at high concentrations of minoxidil (2.55 mg/L-5 mg/L). In addition, a decrease in the mitotic index (8.2) and an increase in the frequency of chromosomal abnormalities (10.2) were observed, suggesting a cytotoxic effect. These findings show that minoxidil, even at low concentrations, can have adverse effects on the morphology and cell division of P. vulgaris. This study demonstrates the potential of plants as tools to evaluate the phytotoxicity and cytotoxicity of drugs and highlights the need to implement measures to reduce the contamination of these drugs in the environment.