Sublethal acetamiprid affects reproduction, development and disrupts gene expression in Binodoxys communis

At present, understanding of neonicotinoid toxicity in arthropods remains limited. We here evaluated the lethal and sublethal effects of acetamiprid in F₀ and F₁ generations of Binodoxys communis using a range of sublethal concentrations. The 10% lethal concentration (LC₁₀) and half lethal concentration (LC₂₅) of ACE had negative effects on the B. communis survival rate, adult longevity, parasitism rate, and emergence rate, and significantly prolonged the duration of the developmental cycle. ACE also had intergenerational effects, with some biological indices affected in the F₁ generation after pesticide exposure. Transcriptomic analysis demonstrated that differentially expressed genes were enriched in specific pathways including the amino acid metabolism, carbohydrate metabolism, energy metabolism, exogenous metabolism, signal transduction, and glutathione metabolism pathways. These results indicated strong contact toxicity of ACE to B. communis, which may inhibit their biological control capacity. These results improve our understanding of the toxicological mechanisms of parasitic natural enemies in response to insecticide exposure.