Background The clinical relevance of <InlineEquation ID="IEq1"> <InlineMediaObject> <ImageObject Color="BlackWhite" FileRef="11332_2025_1387_Article_IEq1.gif" Format="GIF" Height="20" Rendition="HTML" Resolution="72" Type="Linedraw" Width="32" /> </InlineMediaObject> <EquationSource Format="TEX">${\dot{\text{V}}\text{O}}_{2}$</EquationSource> <EquationSource Format="MATHML"><math> <msub> <mrow> <mover accent="true"> <mtext>V</mtext> <mo>˙</mo> </mover> <mtext>O</mtext> </mrow> <mn>2</mn> </msub> </math></EquationSource> </InlineEquation> kinetics is closely associated with the reliability of its corresponding variables and submaximal test outcomes. Purpose The aim of this study was to assess the test–retest reliability of <InlineEquation ID="IEq2"> <InlineMediaObject> <ImageObject Color="BlackWhite" FileRef="11332_2025_1387_Article_IEq2.gif" Format="GIF" Height="20" Rendition="HTML" Resolution="72" Type="Linedraw" Width="32" /> </InlineMediaObject> <EquationSource Format="TEX">${\dot{\text{V}}\text{O}}_{{2}}$</EquationSource> <EquationSource Format="MATHML"><math> <msub> <mrow> <mover accent="true"> <mtext>V</mtext> <mo>˙</mo> </mover> <mtext>O</mtext> </mrow> <mn>2</mn> </msub> </math></EquationSource> </InlineEquation> kinetics in apparently healthy men and women during a 6-min step test (6MST). Methods Twenty-three participants (fifteen men and eight women) performed two free-cadence 6MST on the same day, with a 1-h interval between the tests. Oxygen consumption was monitored using a portable ergospirometer. The following on- and off-kinetic variables were assessed: AO (baseline oxygen uptake); A1 (amplitude of variation); TD (time delay); τ (time constant); MRT (mean response time); R2 (sum of squares of residuals); SSE (sum square errors) Test–retest reliability was analyzed using the intraclass coefficient correlation (ICC), coefficient of variation (CV) and Bland–Altman analysis. Results The ICC, CV (%), and mean difference for <InlineEquation ID="IEq3"> <InlineMediaObject> <ImageObject Color="BlackWhite" FileRef="11332_2025_1387_Article_IEq1.gif" Format="GIF" Height="20" Rendition="HTML" Resolution="72" Type="Linedraw" Width="32" /> </InlineMediaObject> <EquationSource Format="TEX">${\dot{\text{V}}\text{O}}_{2}$</EquationSource> <EquationSource Format="MATHML"><math> <msub> <mrow> <mover accent="true"> <mtext>V</mtext> <mo>˙</mo> </mover> <mtext>O</mtext> </mrow> <mn>2</mn> </msub> </math></EquationSource> </InlineEquation> on- and off-kinetics ranged from 0.57 to 0.97, 5.8% to 9.9% and – 2.2 to 1.7 s, respectively, in men. For women, the corresponding results ranged from 0.59 to 0.95, 5.1% to 15.7% and – 2.9 to 7.2 s, respectively, for ICC, CV (%), and mean difference. Conclusion The analysis of <InlineEquation ID="IEq4"> <InlineMediaObject> <ImageObject Color="BlackWhite" FileRef="11332_2025_1387_Article_IEq2.gif" Format="GIF" Height="20" Rendition="HTML" Resolution="72" Type="Linedraw" Width="32" /> </InlineMediaObject> <EquationSource Format="TEX">${\dot{\text{V}}\text{O}}_{{2}}$</EquationSource> <EquationSource Format="MATHML"><math> <msub> <mrow> <mover accent="true"> <mtext>V</mtext> <mo>˙</mo> </mover> <mtext>O</mtext> </mrow> <mn>2</mn> </msub> </math></EquationSource> </InlineEquation> kinetics during the 6MST demonstrates moderate (0.41–0.60) to excellent (0.81–1.00) reliability indices in men and substantial (0.61–0.80) to excellent (0.81–1.00) reliability in women. In contrast, the analysis of <InlineEquation ID="IEq5"> <InlineMediaObject> <ImageObject Color="BlackWhite" FileRef="11332_2025_1387_Article_IEq2.gif" Format="GIF" Height="20" Rendition="HTML" Resolution="72" Type="Linedraw" Width="32" /> </InlineMediaObject> <EquationSource Format="TEX">${\dot{\text{V}}\text{O}}_{{2}}$</EquationSource> <EquationSource Format="MATHML"><math> <msub> <mrow> <mover accent="true"> <mtext>V</mtext> <mo>˙</mo> </mover> <mtext>O</mtext> </mrow> <mn>2</mn> </msub> </math></EquationSource> </InlineEquation> off-kinetics reveals substantial (0.61–0.80) to excellent (0.81–1.00) reliability in men and moderate (0.41–0.60) to excellent (0.81–1.00) in women, according to ICC classification.

Test–retest reliability of oxygen uptake kinetics-on and -off during 6-min step test in healthy men and women

期刊论文
发表日期：

2025年4月2日

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Renan Shida Marinho,
Aldair Darlan Santos-de-Araújo,
Débora Mayumi de Oliveira Kawakami,
Izadora Moraes Dourado,
Cássia da Luz Goulart,
Daniela Bassi-Dibai,
Meliza Goi Roscani,
Renata Gonçalves Mendes,
Heleen Demeyer,
Audrey Borghi-Silva

摘要

Background

The clinical relevance of ${\dot{\text{V}}\text{O}}_{2}$ ${\dot{V} O}_{2}$ kinetics is closely associated with the reliability of its corresponding variables and submaximal test outcomes.

Purpose

The aim of this study was to assess the test–retest reliability of ${\dot{\text{V}}\text{O}}_{{2}}$ ${\dot{V} O}_{2}$ kinetics in apparently healthy men and women during a 6-min step test (6MST).

Methods

Twenty-three participants (fifteen men and eight women) performed two free-cadence 6MST on the same day, with a 1-h interval between the tests. Oxygen consumption was monitored using a portable ergospirometer. The following on- and off-kinetic variables were assessed: AO (baseline oxygen uptake); A1 (amplitude of variation); TD (time delay); τ (time constant); MRT (mean response time); R² (sum of squares of residuals); SSE (sum square errors) Test–retest reliability was analyzed using the intraclass coefficient correlation (ICC), coefficient of variation (CV) and Bland–Altman analysis.

Results

The ICC, CV (%), and mean difference for ${\dot{\text{V}}\text{O}}_{2}$ ${\dot{V} O}_{2}$ on- and off-kinetics ranged from 0.57 to 0.97, 5.8% to 9.9% and – 2.2 to 1.7 s, respectively, in men. For women, the corresponding results ranged from 0.59 to 0.95, 5.1% to 15.7% and – 2.9 to 7.2 s, respectively, for ICC, CV (%), and mean difference.

Conclusion

The analysis of ${\dot{\text{V}}\text{O}}_{{2}}$ ${\dot{V} O}_{2}$ kinetics during the 6MST demonstrates moderate (0.41–0.60) to excellent (0.81–1.00) reliability indices in men and substantial (0.61–0.80) to excellent (0.81–1.00) reliability in women. In contrast, the analysis of ${\dot{\text{V}}\text{O}}_{{2}}$ ${\dot{V} O}_{2}$ off-kinetics reveals substantial (0.61–0.80) to excellent (0.81–1.00) reliability in men and moderate (0.41–0.60) to excellent (0.81–1.00) in women, according to ICC classification.