<p>The Movement Assessment Battery for Children—second edition (MABC-2) is a commonly used tool for assessing children's motor skills, which are vital for daily activities, motor development, learning readiness, and physical activity engagement. The MABC-2 has been established as an effective tool for monitoring motor development, evaluating motor skills, and screening for atypical development across cultures. However, its applicability to Tunisian children has not been fully investigated. To address this research gap, the present study explored the MABC-2 reliability in Tunisian preschool children (age band 1). Sixty two participants were selected from different kindergartens in Tunisia aged between 3 and 4 years old and 5–6 years old respectively. The results showed promising test–retest reliability across all MABC-2 items within age band 1 as well as between the 3–4 years old and the 5–6 years old groups (intraclass correlation coefficient between 0.7 and 0.99). Internal consistency results showed that all items had Cronbach’s alpha coefficients ranging from 0.65 to 0.79 suggesting that the results yielded by MABC-2 were consistent and reliable. Internal responsiveness revealed that the majority of items had good precision in detecting minor changes in performance, with only two tasks having low sensitivity, necessitating some adjustments to improve the test's psychometric quality. Overall, the MABC-2 demonstrated reliability in assessing motor skills among Tunisian preschoolers, providing valuable insights for clinicians and researchers working in this cultural context.</p>