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Background <p>Controlled-environment cultivation of medicinal cannabis (<i>Cannabis sativa</i> L.) typically optimizes light conditions to enhance the biosynthesis of pharmaceutically important metabolites like cannabinoids. Such experimental strategies may also influence other specialized metabolites like terpenoids, flavonoids, alkaloids, among others. Previous untargeted metabolomics studies testing short-wavelength conditions like UV and blue light have shown that terpenoids and prenylated flavonoids in cannabis leaves respond differentially. However, since metabolomic studies in cannabis have so far mostly focused on floral cannabinoids, a comprehensive untargeted study into cannabis’ floral metabolome response to short wavelengths is currently lacking.</p> Objectives <p>Our study investigates the impact of short-wavelength usage on cannabis specialized metabolism, and in particular the influence of UVB, UVA, and blue light on the cannabis floral flavonoid metabolome and associated glycosylation moieties.</p> Methods <p>Cannabis plants were grown under a white background light and exposed to supplemental UVB, UVA, or blue light during the generative phase of the cultivation cycle. Treatments were compared to a reference white background light without UV or blue light. Metabolites from floral tissue were extracted and analysed via ultra-performance liquid chromatography-tandem mass spectrometry. A comparative metabolomics workflow was designed and used to characterize the floral flavonoid metabolome and associated glycosylation moieties.</p> Results <p>Our results demonstrate how short wavelengths differentially affect the metabolism of natural product compound classes including polyketides and phenylpropanoids/shikimates. Blue light induced flavonoids similarly to how UVB did, while both UVA and blue light specifically induced flavanones accumulation. UVB showed the strongest regulatory effect on flavonoids production and glycosylation patterns.</p> Conclusions <p>UVB reshapes the cannabis floral flavonoid metabolome by selectively stimulating the accumulation and structural modification of flavonoids. Therefore, UVB represents a potential horticultural strategy to enhance flavonoid-related aspects of medicinal cannabis inflorescence phytochemical quality, without affecting cannabinoid levels.</p>

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Characterizing the effect of short wavelengths on the floral flavonoid metabolome of medicinal cannabis using a comparative computational metabolomics workflow

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月1日

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  • Willy Contreras-Avilés,
  • Laura Rosina Torres-Ortega,
  • Ep Heuvelink,
  • Leo F. M. Marcelis,
  • Justin J. J. van der Hooft,
  • Iris F. Kappers

摘要

Background

Controlled-environment cultivation of medicinal cannabis (Cannabis sativa L.) typically optimizes light conditions to enhance the biosynthesis of pharmaceutically important metabolites like cannabinoids. Such experimental strategies may also influence other specialized metabolites like terpenoids, flavonoids, alkaloids, among others. Previous untargeted metabolomics studies testing short-wavelength conditions like UV and blue light have shown that terpenoids and prenylated flavonoids in cannabis leaves respond differentially. However, since metabolomic studies in cannabis have so far mostly focused on floral cannabinoids, a comprehensive untargeted study into cannabis’ floral metabolome response to short wavelengths is currently lacking.

Objectives

Our study investigates the impact of short-wavelength usage on cannabis specialized metabolism, and in particular the influence of UVB, UVA, and blue light on the cannabis floral flavonoid metabolome and associated glycosylation moieties.

Methods

Cannabis plants were grown under a white background light and exposed to supplemental UVB, UVA, or blue light during the generative phase of the cultivation cycle. Treatments were compared to a reference white background light without UV or blue light. Metabolites from floral tissue were extracted and analysed via ultra-performance liquid chromatography-tandem mass spectrometry. A comparative metabolomics workflow was designed and used to characterize the floral flavonoid metabolome and associated glycosylation moieties.

Results

Our results demonstrate how short wavelengths differentially affect the metabolism of natural product compound classes including polyketides and phenylpropanoids/shikimates. Blue light induced flavonoids similarly to how UVB did, while both UVA and blue light specifically induced flavanones accumulation. UVB showed the strongest regulatory effect on flavonoids production and glycosylation patterns.

Conclusions

UVB reshapes the cannabis floral flavonoid metabolome by selectively stimulating the accumulation and structural modification of flavonoids. Therefore, UVB represents a potential horticultural strategy to enhance flavonoid-related aspects of medicinal cannabis inflorescence phytochemical quality, without affecting cannabinoid levels.