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Introduction <p>Selectively-bred green-lipped mussel, <i>Perna canaliculus</i>, families may differ in their physiological responses, potentially altering their sensitivity to preparatory treatment and thereby influencing its effectiveness in mitigating transport stress for live transport.</p> Objective <p>This study investigated whether selectively-bred mussel families differ in their metabolic responses to magnesium chloride (MgCl<sub>2</sub>) pre-treatment for mitigating live transport stress and enhancing recovery.</p> Methods <p>Two full-sibling mussel families with contrasting physiological phenotypes, including a less thermally tolerant family (FamC) and a more thermally tolerant family (FamF), were subjected to MgCl<sub>2</sub> pre-treatment followed by 72-hour simulated live transport and 5-day recovery. Gill metabolic profiles were analysed immediately after transport and during recovery using GC-MS.</p> Results <p>MgCl<sub>2</sub> pre-treatment reduced, but did not eliminate, transport-induced metabolic changes with family-specific responses observed. The less tolerant FamC showed stronger anaerobic metabolic responses to transport stress with 25 differentially expressed metabolites (DEM), resulting in 10 enriched pathways immediately after transport indicating a rapid but energetically costly stress response. In contrast, the more tolerant FamF showed a weaker transport response (12 DEM; 5 pathways) but activated broader metabolic pathways during recovery (21 DEM; 10 pathways), including metabolites linked to cellular protection after 1&#xa0;day of recovery suggesting greater stress resilience and recovery capacity.</p> Conclusion <p>The contrasting metabolic responses between mussel families indicate differences in transport stress resilience and recovery capacity, suggesting that selective breeding could improve resilience and pre-treatments effectiveness during live transport. Such pre-treatment could also be used for research and analytical purposes where minimising transport-induced physiological stress could improve sample quality and experimental reliability.</p>

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Metabolomic insights into inter-familial and pre-transport treatment effects on live transport stress in selectively-bred mussels, Perna canaliculus

  • 期刊论文

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  • 发表日期：

    2026年6月16日

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  • M. C. F. Cheng,
  • L. N. Zamora,
  • N. J. Delorme,
  • N. L. C. Ragg,
  • A. J. R. Hickey,
  • B. J. Dunphy

摘要

Introduction

Selectively-bred green-lipped mussel, Perna canaliculus, families may differ in their physiological responses, potentially altering their sensitivity to preparatory treatment and thereby influencing its effectiveness in mitigating transport stress for live transport.

Objective

This study investigated whether selectively-bred mussel families differ in their metabolic responses to magnesium chloride (MgCl2) pre-treatment for mitigating live transport stress and enhancing recovery.

Methods

Two full-sibling mussel families with contrasting physiological phenotypes, including a less thermally tolerant family (FamC) and a more thermally tolerant family (FamF), were subjected to MgCl2 pre-treatment followed by 72-hour simulated live transport and 5-day recovery. Gill metabolic profiles were analysed immediately after transport and during recovery using GC-MS.

Results

MgCl2 pre-treatment reduced, but did not eliminate, transport-induced metabolic changes with family-specific responses observed. The less tolerant FamC showed stronger anaerobic metabolic responses to transport stress with 25 differentially expressed metabolites (DEM), resulting in 10 enriched pathways immediately after transport indicating a rapid but energetically costly stress response. In contrast, the more tolerant FamF showed a weaker transport response (12 DEM; 5 pathways) but activated broader metabolic pathways during recovery (21 DEM; 10 pathways), including metabolites linked to cellular protection after 1 day of recovery suggesting greater stress resilience and recovery capacity.

Conclusion

The contrasting metabolic responses between mussel families indicate differences in transport stress resilience and recovery capacity, suggesting that selective breeding could improve resilience and pre-treatments effectiveness during live transport. Such pre-treatment could also be used for research and analytical purposes where minimising transport-induced physiological stress could improve sample quality and experimental reliability.