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Introduction <p>Metabolic processes play a role in cancer development, with faecal amino acids emerging as potential biomarkers for colorectal neoplasia. While fresh frozen tissue is preferred for metabolomic analysis, formalin-fixed paraffin-embedded (FFPE) tissue is more widely available.</p> Objectives <p>We aimed to evaluate amino acid profiles in FFPE tissue across different stages of the adenoma-carcinoma sequence.</p> Method <p>A panel of 20 amino acids was measured using liquid chromatography–tandem mass spectrometry.</p> Results <p>Fourteen amino acids were detected, with proline elevated in colorectal carcinoma versus advanced (FC 2.33, <i>p</i> = 0.04) and non-advanced adenomas (FC 2.42, <i>p</i> = 0.02).</p> Conclusion <p>Despite analytical challenges, amino acid profiling in FFPE tissue is feasible.</p>

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Feasibility of amino acid profiling in long-term stored formalin-fixed paraffin-embedded colorectal neoplasia tissue

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月27日

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  • Roza C. M. Opperman,
  • Puck E. Bruchner,
  • Sofie Bosch,
  • Tim G. J. de Meij,
  • Evelien Dekker,
  • Nanne K. H. de Boer,
  • Eduard A. Struys

摘要

Introduction

Metabolic processes play a role in cancer development, with faecal amino acids emerging as potential biomarkers for colorectal neoplasia. While fresh frozen tissue is preferred for metabolomic analysis, formalin-fixed paraffin-embedded (FFPE) tissue is more widely available.

Objectives

We aimed to evaluate amino acid profiles in FFPE tissue across different stages of the adenoma-carcinoma sequence.

Method

A panel of 20 amino acids was measured using liquid chromatography–tandem mass spectrometry.

Results

Fourteen amino acids were detected, with proline elevated in colorectal carcinoma versus advanced (FC 2.33, p = 0.04) and non-advanced adenomas (FC 2.42, p = 0.02).

Conclusion

Despite analytical challenges, amino acid profiling in FFPE tissue is feasible.