Scholarlink

Background <p>Preclinical rodent models are vital in cancer research, and enhancing their relevance reduces animal use.</p> Aim <p>The aim of this pilot study was to determine if A549 lung cancer spheroids implanted into athymic nude mice will change the serum metabolite profile and express serum cancer biomarkers.</p> Method <p>Spheroid non-small cell carcinoma tumours were implanted into athymic nude mice. <sup>1</sup>H-NMR metabolomics was used to evaluate the serum metabolome. Specific cancer biomarkers were determined using commercial kits.</p> Results <p>Tumours produced significant levels of selected serum cancer biomarkers, and the implanted tumours had changed the serum metabolite profiles—mimicking human lung cancer profiles—which were significantly different from the non-cancer control. Histological analysis confirmed characteristics of solid adenocarcinomas.</p> Conclusion <p>The A549 spheroid-derived xenograft model demonstrates promise as a feasible and physiologically relevant tool for non-small cell lung cancer research.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Assessing metabolic alterations and cancer biomarkers in athymic nude mice implanted with NSCLC spheroids

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年6月11日

查看全文
  • Charity Mabela,
  • Chrisna Gouws,
  • Shayne Mason,
  • Wihan Pheiffer

摘要

Background

Preclinical rodent models are vital in cancer research, and enhancing their relevance reduces animal use.

Aim

The aim of this pilot study was to determine if A549 lung cancer spheroids implanted into athymic nude mice will change the serum metabolite profile and express serum cancer biomarkers.

Method

Spheroid non-small cell carcinoma tumours were implanted into athymic nude mice. 1H-NMR metabolomics was used to evaluate the serum metabolome. Specific cancer biomarkers were determined using commercial kits.

Results

Tumours produced significant levels of selected serum cancer biomarkers, and the implanted tumours had changed the serum metabolite profiles—mimicking human lung cancer profiles—which were significantly different from the non-cancer control. Histological analysis confirmed characteristics of solid adenocarcinomas.

Conclusion

The A549 spheroid-derived xenograft model demonstrates promise as a feasible and physiologically relevant tool for non-small cell lung cancer research.