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Introduction <p>Pyridoxal 5’-phosphate (PLP), the biologically active form of vitamin B6 is involved in 4% of cellular enzymatic activities and its deficiency is responsible for or contributes to several human diseases. The study of underlying mechanisms is still in its infancy and requires suitable model organisms. In <i>Drosophila</i> the deficiency of vitamin B6 produces chromosome aberrations and hallmarks of human diseases including diabetes and cancer. However, the effects of vitamin B6 deficiency have never been examined at a metabolic level.</p> Objectives <p>This study evaluates the metabolic changes in vitamin B6 deficient <i>Drosophila</i> larvae with the aim of validating flies as a suitable model for diseases associated to vitamin B6 deficiency.</p> Methods <p>To induce vitamin B6 deficiency we fed <i>Drosophila</i> wild type larvae with 4-deoxypyridoxine (4DP), a PLP antagonist. By HPLC analysis we verified that the 4DP treatment was effective in inducing vitamin B6 deficiency. Using an NMR-based metabolomic approach we compared the metabolites in larval extracts from untreated and 4DP-fed larvae.</p> Results <p>The NMR spectra analysis identified quantitative differences for sixteen metabolites out of forty, including branched chain and aromatic amino acids, glucose, and lipids, thus revealing interesting possible associations with the phenotypes showed by vitamin B6 deficient flies.</p> Conclusions <p>Our results validate <i>Drosophila</i> as a suitable model to study in depth the molecular mechanisms underlying human diseases associated with vitamin B6 deficiency and confirmed that 4DP treatment is effective in inducing vitamin B6 deficiency.</p>

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Vitamin B6 deficiency produces metabolic alterations in Drosophila

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年3月23日

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  • Giulia Tesoriere,
  • Eleonora Pilesi,
  • Michele De Rosa,
  • Ottavia Giampaoli,
  • Adriano Patriarca,
  • Mariangela Spagnoli,
  • Federica Chiocciolini,
  • Angela Tramonti,
  • Roberto Contestabile,
  • Fabio Sciubba,
  • Fiammetta Vernì

摘要

Introduction

Pyridoxal 5’-phosphate (PLP), the biologically active form of vitamin B6 is involved in 4% of cellular enzymatic activities and its deficiency is responsible for or contributes to several human diseases. The study of underlying mechanisms is still in its infancy and requires suitable model organisms. In Drosophila the deficiency of vitamin B6 produces chromosome aberrations and hallmarks of human diseases including diabetes and cancer. However, the effects of vitamin B6 deficiency have never been examined at a metabolic level.

Objectives

This study evaluates the metabolic changes in vitamin B6 deficient Drosophila larvae with the aim of validating flies as a suitable model for diseases associated to vitamin B6 deficiency.

Methods

To induce vitamin B6 deficiency we fed Drosophila wild type larvae with 4-deoxypyridoxine (4DP), a PLP antagonist. By HPLC analysis we verified that the 4DP treatment was effective in inducing vitamin B6 deficiency. Using an NMR-based metabolomic approach we compared the metabolites in larval extracts from untreated and 4DP-fed larvae.

Results

The NMR spectra analysis identified quantitative differences for sixteen metabolites out of forty, including branched chain and aromatic amino acids, glucose, and lipids, thus revealing interesting possible associations with the phenotypes showed by vitamin B6 deficient flies.

Conclusions

Our results validate Drosophila as a suitable model to study in depth the molecular mechanisms underlying human diseases associated with vitamin B6 deficiency and confirmed that 4DP treatment is effective in inducing vitamin B6 deficiency.