Plasma metabolites in childhood Burkitt lymphoma cases and cancer-free controls in Uganda

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2024年6月28日

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Jiaqi Huang,
Hadijah Nabalende,
M. Constanza Camargo,
Jacqueline Lovett,
Isaac Otim,
Ismail D. Legason,
Martin D. Ogwang,
Patrick Kerchan,
Tobias Kinyera,
Leona W. Ayers,
Kishor Bhatia,
James J. Goedert,
Steven J. Reynolds,
Peter D. Crompton,
Steven C. Moore,
Ruin Moaddel,
Demetrius Albanes,
Sam M. Mbulaiteye

摘要

Introduction

Burkitt lymphoma (BL) is an aggressive non-Hodgkin lymphoma associated with Plasmodium falciparum and Epstein-Barr virus, both of which affect metabolic pathways. The metabolomic patterns of BL is unknown.

Materials and methods

We measured 627 metabolites in pre-chemotherapy treatment plasma samples from 25 male children (6–11 years) with BL and 25 cancer-free area- and age-frequency-matched male controls from the Epidemiology of Burkitt Lymphoma in East African Children and Minors study in Uganda using liquid chromatography-tandem mass spectrometry. Unconditional, age-adjusted logistic regression analysis was used to estimate odds ratios (ORs) and their 95% confidence intervals (CIs) for the BL association with 1-standard deviation increase in the log-metabolite concentration, adjusting for multiple comparisons using false discovery rate (FDR) thresholds and Bonferroni correction.

Results

Compared to controls, levels for 42 metabolite concentrations differed in BL cases (FDR < 0.001), including triacylglyceride (18:0_38:6), alpha-aminobutyric acid (AABA), ceramide (d18:1/20:0), phosphatidylcholine ae C40:6 and phosphatidylcholine C38:6 as the top signals associated with BL (ORs = 6.9 to 14.7, P < 2.4✕10^− 4). Two metabolites (triacylglyceride (18:0_38:6) and AABA) selected using stepwise logistic regression discriminated BL cases from controls with an area under the curve of 0.97 (95% CI: 0.94, 1.00).

Conclusion

Our findings warrant further examination of plasma metabolites as potential biomarkers for BL risk/diagnosis.