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<p>Telomeres are special chromatin structures located at the ends of eukaryotic chromosomes. The elongation and shortening of telomeres during plant growth and development are associated with cell replication, differentiation and regeneration. The shortening of telomeres is considered one of the most important biomarkers of organismal senescence. However, further clarification is required regarding the relationship between telomere length and tree longevity in long‒lived tree species. <i>Platycladus orientalis</i> (L.) Franco, China’s peculiar Cupressaceae plant, has the largest distribution of ancient trees in the world. To elucidate the relationships between telomere length and telomere‒associated proteins, as well as the lifespan of <i>P. orientalis</i>, 12 <i>P. orientalis</i> telomere‒associated proteins were initially screened on the basis of the third–generation full–length transcriptome sequencing of <i>P. orientalis</i>. Based on this, we investigated the relationships among the relative telomere‒associated protein‒encoding gene expression and tree age and the relative telomere length among <i>P. orientalis</i> individuals ranging in age from 2 age (a) to 2000a. The results revealed that: (1) the full‒length transcriptome yielded a total of 20,107,710 subreads, 49,857 coding sequences (CDSs). And 12 telomere‒associated proteins, namely, WHY1, WHY2, TRB1, TRB2, TRB3, STEP1, RAD50, KU70, TBP1, TRP3, TELO2, and TRP6, were screened. (2) The relative telomere length was negatively correlated with tree age in leaves from younger <i>P. orientalis</i> individuals (10‒200a), whereas increased slightly in leaves from older individuals (500‒2000a). The relative telomere length in phloem tissues decreased significantly with age, except at 200a. The relative expression of telomere‒associated protein‒encoding genes was positively correlated with the relative telomere length in leaves from younger individuals and phloem tissues from older individuals, except for <i>KU70</i>.</p>

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Analysis of the relationships among telomere‒associated protein‒encoding gene expression, tree age and telomere length in Platycladus orientalis (L.) Franco

  • 期刊论文

  • 发表日期：

    2025年5月10日

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  • Yaru Zhang,
  • Xuan Yu,
  • Sheng Zhang

摘要

Telomeres are special chromatin structures located at the ends of eukaryotic chromosomes. The elongation and shortening of telomeres during plant growth and development are associated with cell replication, differentiation and regeneration. The shortening of telomeres is considered one of the most important biomarkers of organismal senescence. However, further clarification is required regarding the relationship between telomere length and tree longevity in long‒lived tree species. Platycladus orientalis (L.) Franco, China’s peculiar Cupressaceae plant, has the largest distribution of ancient trees in the world. To elucidate the relationships between telomere length and telomere‒associated proteins, as well as the lifespan of P. orientalis, 12 P. orientalis telomere‒associated proteins were initially screened on the basis of the third–generation full–length transcriptome sequencing of P. orientalis. Based on this, we investigated the relationships among the relative telomere‒associated protein‒encoding gene expression and tree age and the relative telomere length among P. orientalis individuals ranging in age from 2 age (a) to 2000a. The results revealed that: (1) the full‒length transcriptome yielded a total of 20,107,710 subreads, 49,857 coding sequences (CDSs). And 12 telomere‒associated proteins, namely, WHY1, WHY2, TRB1, TRB2, TRB3, STEP1, RAD50, KU70, TBP1, TRP3, TELO2, and TRP6, were screened. (2) The relative telomere length was negatively correlated with tree age in leaves from younger P. orientalis individuals (10‒200a), whereas increased slightly in leaves from older individuals (500‒2000a). The relative telomere length in phloem tissues decreased significantly with age, except at 200a. The relative expression of telomere‒associated protein‒encoding genes was positively correlated with the relative telomere length in leaves from younger individuals and phloem tissues from older individuals, except for KU70.