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<p>To explore functional biological control resources for the sustainable management of <i>Pinus armandii</i> blister rust, this study screened endophytic bacteria with superior plant growth-promoting (PGP) and antagonistic functions to construct a functionally complementary and stable synthetic microbial community (SynCom). Endophytic bacteria were isolated from multiple tissues of <i>P. armandii</i> and assessed for PGP traits, including nitrogen-fixing potential, phosphate and potassium solubilization, and indole-3-acetic acid (IAA) production, as well as antagonistic potential using dual-culture assays and inhibition of urediniospore germination. Based on pairwise compatibility tests, a SynCom was constructed, and its constituent strains were phylogenetically assigned based on 16&#xa0;S rRNA and <i>gyr</i>A gene sequencing, and evaluated in pot experiments for its effects on seedling growth and blister rust-associated defense responses. A total of 112 cultivable isolates were obtained, yielding four multifunctional strains and nine strains with significant antagonistic activity. The optimized SynCom, comprising <i>Pseudomonas koreensis</i> RC1, <i>Paenibacillus terrae</i> RE7, <i>Bacillus velezensis</i> OB3 and NA3, and <i>Bacillus subtilis</i> NA11, significantly increased seedling height and biomass accumulation. In addition, SynCom treatment enhanced defense-related enzyme activities, increasing polyphenol oxidase (PPO) and peroxidase (POD) activities by 19.1% and 157.3% relative to the control, respectively. Overall, the constructed SynCom integrates growth promotion with enhanced defense-associated responses, providing a basis for subsequent in planta evaluation of its biocontrol potential against <i>P. armandii</i> blister rust.</p>

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Establishment of a functional endophytic bacterial synthetic community for growth promotion and blister rust-associated defense responses in Pinus armandii

  • 期刊论文

  • 发表日期：

    2026年6月20日

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  • Yujie Xing,
  • Xinyi Zhang,
  • Xinye Liu,
  • Zhongdong Yu

摘要

To explore functional biological control resources for the sustainable management of Pinus armandii blister rust, this study screened endophytic bacteria with superior plant growth-promoting (PGP) and antagonistic functions to construct a functionally complementary and stable synthetic microbial community (SynCom). Endophytic bacteria were isolated from multiple tissues of P. armandii and assessed for PGP traits, including nitrogen-fixing potential, phosphate and potassium solubilization, and indole-3-acetic acid (IAA) production, as well as antagonistic potential using dual-culture assays and inhibition of urediniospore germination. Based on pairwise compatibility tests, a SynCom was constructed, and its constituent strains were phylogenetically assigned based on 16 S rRNA and gyrA gene sequencing, and evaluated in pot experiments for its effects on seedling growth and blister rust-associated defense responses. A total of 112 cultivable isolates were obtained, yielding four multifunctional strains and nine strains with significant antagonistic activity. The optimized SynCom, comprising Pseudomonas koreensis RC1, Paenibacillus terrae RE7, Bacillus velezensis OB3 and NA3, and Bacillus subtilis NA11, significantly increased seedling height and biomass accumulation. In addition, SynCom treatment enhanced defense-related enzyme activities, increasing polyphenol oxidase (PPO) and peroxidase (POD) activities by 19.1% and 157.3% relative to the control, respectively. Overall, the constructed SynCom integrates growth promotion with enhanced defense-associated responses, providing a basis for subsequent in planta evaluation of its biocontrol potential against P. armandii blister rust.