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<p>Whole-genome sequencing (WGS) of microbial isolates is a valuable tool for mapping the genomes of novel organisms and is helpful for understanding plant-bacteria interactions. The close relationships between bacteria and plants are essential for maintaining healthy ecosystems, whether the bacteria reside the plant or in the rhizosphere surrounding its roots. In this study, isolation, characterization, and WGS were performed to identify promising plant growth-promoting rhizobacteria (PGPR) using the rhizospheric soil sample of jatropha tree roots. Out of 100 isolates, six (FACU 2024, 2, 3, 4, 5, and 6) exhibited phosphate-solubilizing bacteria (PSB) traits, including solubilizing phosphate and producing indole acetic acid (IAA), and the ability of other plant growth-promoting (PGP) traits was tested. Isolate FACU 2024 exhibited the highest values for IAA production (12.1&#xa0;µg/ml), soluble phosphate release (300&#xa0;µg/ml), and phosphate solubilization index (6.7). Therefore, FACU 2024 was molecularly identified as <i>Escherichia coli.</i> The WGS analysis revealed that <i>E. coli</i> FACU 2024 possesses one chromosome and one plasmid with a total length of 4.8&#xa0;Mb and were submitted on GenBank under accession numbers CP147009 and CP147010. The bacterial genome contained about 142 PGP genes, ranging from 258 to 3744&#xa0;bp and associated with phosphate solubilization, siderophore production, indole acetic acid (IAA) production, nitrogen metabolism, nitrogen fixation, and nitrite/nitrate reduction. Moreover, genomic islands (GIs) were enriched with genes associated with horizontal gene transfer (HGT), stress response, and environmental adaptation, and prophage analyses were carried out. In addition, 15 heavy metal resistance genes were annotated, such as those for As, Cd, Zn, Pb, Cu, Fe, and Co, ranging from 426 to 2505&#xa0;bp. This study provides the first comprehensive genetic evidence linking <i>E. coli</i> to key PGPR traits alongside genes conferring resistance to multiple heavy metals. This strain demonstrates potential as a PGPR in addition to heavy metal bioremediation.</p>

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Genome-based characterization of a multifunctional plant growth-promoting and heavy metal-resistant Escherichia coli FACU2024 isolated from Jatropha rhizosphere

  • 期刊论文

  • 发表日期：

    2026年5月25日

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  • Asmaa A. Halema,
  • Nagwa I. Elarabi,
  • Ahmed R. Henawy,
  • Hayfa Habes Almutairi,
  • Hossam El-Beltagi,
  • Othman Al-Dossary,
  • Bader Alsubaie,
  • Adel A. Rezk,
  • Abdelhadi A. Abdelhadi,
  • Heba A.R. Abdelhaleem

摘要

Whole-genome sequencing (WGS) of microbial isolates is a valuable tool for mapping the genomes of novel organisms and is helpful for understanding plant-bacteria interactions. The close relationships between bacteria and plants are essential for maintaining healthy ecosystems, whether the bacteria reside the plant or in the rhizosphere surrounding its roots. In this study, isolation, characterization, and WGS were performed to identify promising plant growth-promoting rhizobacteria (PGPR) using the rhizospheric soil sample of jatropha tree roots. Out of 100 isolates, six (FACU 2024, 2, 3, 4, 5, and 6) exhibited phosphate-solubilizing bacteria (PSB) traits, including solubilizing phosphate and producing indole acetic acid (IAA), and the ability of other plant growth-promoting (PGP) traits was tested. Isolate FACU 2024 exhibited the highest values for IAA production (12.1 µg/ml), soluble phosphate release (300 µg/ml), and phosphate solubilization index (6.7). Therefore, FACU 2024 was molecularly identified as Escherichia coli. The WGS analysis revealed that E. coli FACU 2024 possesses one chromosome and one plasmid with a total length of 4.8 Mb and were submitted on GenBank under accession numbers CP147009 and CP147010. The bacterial genome contained about 142 PGP genes, ranging from 258 to 3744 bp and associated with phosphate solubilization, siderophore production, indole acetic acid (IAA) production, nitrogen metabolism, nitrogen fixation, and nitrite/nitrate reduction. Moreover, genomic islands (GIs) were enriched with genes associated with horizontal gene transfer (HGT), stress response, and environmental adaptation, and prophage analyses were carried out. In addition, 15 heavy metal resistance genes were annotated, such as those for As, Cd, Zn, Pb, Cu, Fe, and Co, ranging from 426 to 2505 bp. This study provides the first comprehensive genetic evidence linking E. coli to key PGPR traits alongside genes conferring resistance to multiple heavy metals. This strain demonstrates potential as a PGPR in addition to heavy metal bioremediation.